Objective: To quantitatively measure the expression of low-density lipoprotein receptor-related protein 1B gene (LRP1B) in normal and cervical squamous cell carcinoma tissues and explore the correlation between LRP1B gene and age, degree of tumor differentiation, clinical staging, degree of infiltration, lymph node metastasis and the infection of human papilloma virus (HPV) 16/18.
Methods: Forty patients diagnosed with cervical squamous cell carcinoma and 20 healthy subjects were recruited. The expression of LRP1B mRNA was quantitatively measured by in situ hybridization using LRP1B oligonucleotide probe. The expression of HPV16/18 in the cervical squamous cell carcinoma specimen was investigated.
Results: Among 40 cervical carcinoma samples, the expression level of LRP1B mRNA was down-regulated in 22. Among 20 healthy controls, low expression of LRP1B mRNA was observed in 3. The negative rate of LRP1B mRNA expression in the cervical carcinoma group was significantly lower compared with that in the normal tissues (P<0.05). The positive rate of HPV16/18 in patients with negative LRP1B was significantly higher than that in their counterparts positive for LRP1B (P<0.05). In the cervical carcinoma group, the expression of LRP1B mRNA was not correlated with age, degree of tumor differentiation or clinical staging (all P>0.05). The expression level of LRP1B mRNA in patients with serous membrane infiltration and lymph node metastasis was significantly down-regulated (both P<0.05).
Conclusion: LRP1B is lowly expressed in cervical squamous cell carcinoma tissues, suggests LRP1B gene probably acts as a new tumor suppressor gene. LRP1B gene alteration and HPV16/18 infection play a coordinating role in the incidence of cervical cancer. LRP1B expression is intimately correlated with the incidence and metastasis of cervical squamous cell carcinoma.
Keywords: Cervical squamous cell cancer; HPV16/18; LRP1B; in situ hybridization.
IJCEP Copyright © 2018.