miR-5582-5p inhibits cell proliferation of non-small cell lung cancer through targeting FGF-10

Tao Han; Yabo Mei; Yan Wang; Zhichun Feng

miR-5582-5p inhibits cell proliferation of non-small cell lung cancer through targeting FGF-10

Int J Clin Exp Pathol. 2019 Mar 1;12(3):1087-1094. eCollection 2019.

Authors

Tao Han¹, Yabo Mei², Yan Wang², Zhichun Feng¹

Affiliations

¹ The Clinical Medical College, The Seventh Medical Center of PLA General Hospital, The Second Military Medical University of PLA Beijing, China.
² Affiliated BaYi Children's Hospital, The Seventh Medical Center of PLA General Hospital Beijing, China.

PMID: 31933923
PMCID: PMC6945143

Abstract

Accumulating evidence has suggested that microRNAs (miRNAs) play important roles in regulating the progression of cancerby acting as tumor suppressors or oncogenes. Here, our results demonstrated that miR-5582-5p was significantly down-regulated in non-small cell lung cancer (NSCLC) tissues and cell lines compared with normal controls. Overexpression of miR-5582-5p markedly inhibited the proliferation and migration of NSCLC cells. Consistently, the apoptosis of NSCLC cells was also significantly promoted by overexpressed miR-5582-5p. Functional study uncovered that miR-5582-5p bound the 3'-untranslated region (UTR) of fibroblastic growth factor-10 (FGF-10) and decreased the expression of FGF-10 in NSCLC cells. FGF-10 was up-regulated in NSCLC tissues and inversely correlated with the level of miR-5582-5p in NSCLC tissues. Overexpression of FGF-10 significantly reversed the inhibitory effect of miR-5582-5p on the proliferation of NSCLC cells. Taken together, our results demonstrated the functional mechanism of miR-5582-5p in suppressing malignant behaviors of NSCLC cells by targeting FGF-10. These findings demonstrated that miR-5582-5p might be a novel therapeutic target in the treatment of NSCLC.

Keywords: FGF-10; NSCLC; miR-5582-5p.