Background: Ovarian cancer is one of the six most common women's cancers in the world, and is found among the top three female genital tumors, which is lower than the rate of endometrial cancer and cervical cancer.
Methods: This study utilized miR-675 mimics and siRNA transfection to construct miR-675 overexpression and lower-expression model to investigate the effect of miR-675 on the regulation of ovarian cancer. Western blotting and RT-qPCR were applied for the quantitative testing of mRNA and protein expression. Apoptosis of podocytes was detected by TUNEL staining.
Results: H19 expression was also up-regulated. In vitro, H19 silencing after transfection with si-H19 could suppress proliferation and invasion. Luciferase reporter assay revealed a close link between miR-675 and H19 3'-UTR. Furthermore, combining experiments of miR-675 and H19 indicated that miR-675 could reverse the function of H19 on ovarian cancer.
Conclusion: Our study reveals the overexpression of H19 in ovarian cancer tissue and cells, and discovers an oncogenic role in ovarian cancer via sponging miR-675, providing a potential biomarker for early detection and prognosis of ovarian cancer.
Keywords: H19; Long non-coding RNA; cell proliferation; miR-675.
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