Enzyme immobilization is a widespread empiric technology to achieve more stable, active and reusable enzymes. The empiricism can be reduced by the application of rational design procedures employing bioinformatic tools, engineered-proteins and detailed analyses of existent data. In this chapter, we describe relevant approaches to rationalize the design of enzyme immobilization protocols, with special attention to the modulation of immobilization pH to regulate the operational stability of glutaraldehyde cross-linked enzymes and the coating of iron-containing supports to preserve the integrity of iron-sensitive enzymes. Other strategies, such as the use of factorial planning, optimization of specific enzyme orientation through protein engineering and the use of mathematical algorithms and in silico prediction tools are also described to reduce the classical empiricism. Finally, a public repository creation is proposed as a new promising tool to develop an improvement on future rational design procedures of enzyme immobilization.
Keywords: Empiricism; Enzyme immobilization; Enzyme immobilization in silico prediction; Factorial planning; Glutaraldehyde cross-linking; Iron-sensitive enzymes; Rationalization of protocols; Site-specific immobilization.
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