Abstract
Gamma-aminobutyric acid (GABA) is produced by Lactobacillus brevis using date residue fermentation. In this study, the GABA production method was improved, for which L. brevis strain JCM 1059T was the most efficient among the four L. brevis strains examined. This was presumably due to a difference in the expression level of the gene encoding glutamate decarboxylase that catalyzes GABA synthesis.Abbreviation: GABA: gamma-aminobutyric acid.
Keywords:
Gamma-aminobutyric acid; date residue extract; gene expression analysis; glutamate decarboxylase; lactobacillus brevis.
MeSH terms
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Amino Acid Sequence
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Bacterial Proteins / metabolism
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Fermentation
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Gene Expression Regulation, Bacterial
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Genes, Bacterial / genetics
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Glutamate Decarboxylase / genetics*
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Glutamate Decarboxylase / metabolism
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Hydrogen-Ion Concentration
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Levilactobacillus brevis / enzymology*
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Levilactobacillus brevis / genetics*
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Phoeniceae / chemistry*
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Plant Extracts / metabolism*
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RNA, Ribosomal, 16S / genetics
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Reverse Transcriptase Polymerase Chain Reaction
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gamma-Aminobutyric Acid / metabolism*
Substances
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Bacterial Proteins
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Plant Extracts
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RNA, Ribosomal, 16S
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gamma-Aminobutyric Acid
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Glutamate Decarboxylase