Novel feature extraction approach for achieving potential spectral resolution: Green analytical application on zofenopril calcium and hydrochlorothiazide in their spectrally overlapping binary mixture

Hayam M Lotfy; Hany H Monir; Nevin Erk; Yasmin Rostom

doi:10.1016/j.saa.2019.117998

Novel feature extraction approach for achieving potential spectral resolution: Green analytical application on zofenopril calcium and hydrochlorothiazide in their spectrally overlapping binary mixture

Spectrochim Acta A Mol Biomol Spectrosc. 2020 Apr 5:230:117998. doi: 10.1016/j.saa.2019.117998. Epub 2020 Jan 7.

Authors

Hayam M Lotfy¹, Hany H Monir², Nevin Erk³, Yasmin Rostom⁴

Affiliations

¹ Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini Street, Cairo, Egypt; Pharmaceutical Chemistry Department, Faculty of Pharmaceutical Science and Pharmaceutical Industries, Future University in Egypt, Cairo, Egypt.
² Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini Street, Cairo, Egypt.
³ Analytical Chemistry Department, Faculty of Pharmacy, Ankara University, Ankara, Turkey.
⁴ Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini Street, Cairo, Egypt. Electronic address: yasmin.rostom@pharma.cu.edu.eg.

PMID: 31931351
DOI: 10.1016/j.saa.2019.117998

Abstract

A novel and green spectrophotometric method, namely constant extraction, based on extracting a spectral feature value (constant ratio) has been developed and validated for simultaneous estimation of a binary mixture of zofenopril calcium (ZOF) and hydrochlorothiazide (HCT) in their bulk and marketable formulation. A comparative study between this newly developed method and four long-established ones; ratio difference, ratio subtraction coupled with constant multiplication, advanced amplitude modulation and absorbance subtraction has been carried out giving very promising results. Various analytical performance parameters like linearity, accuracy, precision, and robustness were investigated in accordance with ICH (Q2B). Satisfying optimized instrumental parameters; absorbance of ZOF and HCT were linearly increased for the previously mentioned methods in a concentration range 5.0-35.0 and 3.0-20.0 μg/mL, respectively showing correlation coefficients ≥0.9990. Moreover, specificity has been checked through analyzing synthetic mixtures of the studied analytes. Feasibility has been successfully assessed by simultaneous quantification of both analytes in the commercially available formulation. As well, validity was examined and no interference from common excipients was noticed. Observed data has been statistically compared with those of the published one concluding that no significant variations between both results have been indicated. Furthermore, greenness profile of these methods was assessed by analytical Eco-Scale and found superior to that of the reported HPLC method as an even greener approach for the simultaneous analysis of ZOF and HCT.

Keywords: Absorbance subtraction; Advanced amplitude modulation; Constant extraction; Constant multiplication; Green analytical application; Hydrochlorothiazide; Ratio difference; Ratio subtraction and zofenopril calcium.