Abstract
RcnR is a transcription factor that regulates the homeostasis of cobalt and nickel in bacterial cells. Escherichia coli RcnR was crystallized with DNA that encompasses the DNA-binding site. X-ray diffraction data were collected to 2.9 Å resolution. The crystal belonged to space group P6122 or P6522, with unit-cell parameters a = b = 73.59, c = 157.66 Å, α = β = 90, γ = 120°.
Keywords:
RcnR; cobalt homeostasis; nickel homeostasis; transcription factor.
MeSH terms
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Cobalt / chemistry*
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Cobalt / metabolism
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Crystallography, X-Ray
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DNA
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Escherichia coli / chemistry
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Escherichia coli / genetics
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Escherichia coli Proteins / chemistry*
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Escherichia coli Proteins / isolation & purification
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Models, Molecular
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Nickel / chemistry*
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Nickel / metabolism
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Repressor Proteins / chemistry*
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Repressor Proteins / isolation & purification
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X-Ray Diffraction
Substances
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Escherichia coli Proteins
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RcnR protein, E coli
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Repressor Proteins
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Cobalt
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Nickel
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DNA