Changes in circulating endothelial microvesicles in men after myocardial infarction

Vytautas Žėkas; Rėda Matuzevičienė; Dovilė Karčiauskaitė; Dalius Vitkus; Mantas Radzevičius; Aušra Janiulionienė; Aušra Linkevičiūtė; Sandra Kutkienė; Zita Kučinskienė

doi:10.1016/j.advms.2019.12.002

Changes in circulating endothelial microvesicles in men after myocardial infarction

Adv Med Sci. 2020 Mar;65(1):120-126. doi: 10.1016/j.advms.2019.12.002. Epub 2020 Jan 8.

Authors

Vytautas Žėkas¹, Rėda Matuzevičienė², Dovilė Karčiauskaitė², Dalius Vitkus², Mantas Radzevičius², Aušra Janiulionienė³, Aušra Linkevičiūtė², Sandra Kutkienė⁴, Zita Kučinskienė²

Affiliations

¹ Department of Physiology, Biochemistry, Microbiology and Laboratory Medicine, Institute of the Biomedical Sciences, Faculty of Medicine, Vilnius University, Vilnius, Lithuania; Center of Laboratory Medicine, Vilnius University Hospital Santaros Clinics, Vilnius, Lithuania. Electronic address: vytautas.zekas@mf.vu.lt.
² Department of Physiology, Biochemistry, Microbiology and Laboratory Medicine, Institute of the Biomedical Sciences, Faculty of Medicine, Vilnius University, Vilnius, Lithuania; Center of Laboratory Medicine, Vilnius University Hospital Santaros Clinics, Vilnius, Lithuania.
³ Center of Laboratory Medicine, Vilnius University Hospital Santaros Clinics, Vilnius, Lithuania.
⁴ Clinic of Cardiac and Vascular Diseases, Institute of Clinical Medicine, Faculty of Medicine, Vilnius University, Vilnius, Lithuania.

PMID: 31927269
DOI: 10.1016/j.advms.2019.12.002

Abstract

Purpose: The objective of the study was to determine the differences in the numbers of endothelial microvesicles (EMV) after myocardial infarction (MI) and their association with oxidative stress.

Materials and methods: We included 15 post MI patients and 28 healthy controls. Samples were analysed by flow cytometry. We examined four EMV populations: 1) CD144+, CD42a-, CD61^-, 2) CD144+, CD42a+, CD61^-, 3) CD105+, CD42a-, CD61^-and 4) CD31⁺, CD42a-, CD61^-and determined a percentage of CD62e + EMV. Malondialdehyde concentration was determined by ultra-high performance liquid chromatography.

Results: The median of EMV counts differed between controls and patients in: CD105+ (10.91 microvesicles/μl vs. 33.68 microvesicles/μl, P = 0.006), CD144+, CD42a+ (312.87 microvesicles/μl vs. 73.29 microvesicles/μl, P < 0.001) and CD31⁺ (2 microvesicles/μl vs. 1.38 microvesicles/μl, P = 0.021). The median of percentage of CD62e expression differed between controls and patients in: CD105+ (1.35% vs. 14.8%, P < 0.001), CD144+, CD42a+ (56.45% vs. 98.99%, P < 0.001) and CD144+, CD42a- (173.03% vs. 215.56%) EMV. In patients, EMV counts correlated with low-density lipoprotein cholesterol (LDL-C), total cholesterol (TC) and high-density lipoprotein cholesterol (HDL-C) concentrations: CD105+: R = -0.69, P = 0.004 (LDL-C), R = -0.64, P = 0.01 (TC); CD144+, CD42a-: R = -0.68, P = 0.005 (LDL-C), R = -0.63, P = 0.011 (TC); CD144+: R = -0.54, P = 0.038 (HDL-C) and CD144+, CD42a-, CD62e+: R = 0.78, P = 0.001 (HDL-C). In controls, HDL-C concentration correlated with CD105+ (R = -0.395, P = 0.038) and CD105+, CD62e+ (R = -0.716, P < 0.001) counts. Malondialdehyde concentration correlated with CD144+, CD42a- (P = 0.01, R = 0.48) and CD105+, CD62e+ (P = 0.012, R = 0.47) counts.

Conclusions: Changes in EMV levels after the MI period were observed. Counts of EMV and their CD62e expression correlated with dyslipidaemia and oxidative stress.

Keywords: Endothelial microvesicles; Flow cytometry; Myocardial infarction; Oxidative stress.

MeSH terms

Adult
Biomarkers / metabolism*
Case-Control Studies
Cell-Derived Microparticles / metabolism
Cell-Derived Microparticles / pathology*
Endothelial Cells / metabolism
Endothelial Cells / pathology*
Female
Follow-Up Studies
Humans
Male
Middle Aged
Myocardial Infarction / metabolism
Myocardial Infarction / pathology*
Oxidative Stress*
Prognosis
ROC Curve

Substances

Biomarkers