A TaqMan™-based quantitative PCR screening assay for the probiotic Streptococcus salivarius K12 based on the specific detection of its megaplasmid-associated salivaricin B locus

Peter Reid; Nicholas C K Heng; John D Hale; Deepti Krishnan; Julian Crane; John R Tagg; Trudy J Milne

doi:10.1016/j.mimet.2020.105837

A TaqMan™-based quantitative PCR screening assay for the probiotic Streptococcus salivarius K12 based on the specific detection of its megaplasmid-associated salivaricin B locus

J Microbiol Methods. 2020 Mar:170:105837. doi: 10.1016/j.mimet.2020.105837. Epub 2020 Jan 7.

Authors

Peter Reid¹, Nicholas C K Heng¹, John D Hale², Deepti Krishnan², Julian Crane³, John R Tagg², Trudy J Milne⁴

Affiliations

¹ Sir John Walsh Research Institute, Faculty of Dentistry, University of Otago, Dunedin, New Zealand.
² Blis Technologies Limited, 81 Glasgow Street, South Dunedin 9012, New Zealand.
³ School of Medicine and Health Sciences, University of Otago, Wellington, New Zealand.
⁴ Sir John Walsh Research Institute, Faculty of Dentistry, University of Otago, Dunedin, New Zealand. Electronic address: trudy.milne@otago.ac.nz.

PMID: 31923428
DOI: 10.1016/j.mimet.2020.105837

Abstract

In order to assess the colonization efficacy of the oral probiotic Streptococcus salivarius K12, a rapid method for specific detection and enumeration of the strain was developed. Here, we describe a two-step TaqMan™ quantitative PCR assay using primer-probe combinations targeting genes of the locus encoding the lantibiotic bacteriocin salivaricin B.

Keywords: Salivaricin B; Streptococcus salivarius K12; TaqMan™ quantitative PCR assay.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Load / methods*
Bacterial Proteins / genetics
Humans
Plasmids / genetics
Probiotics
Real-Time Polymerase Chain Reaction
Streptococcus salivarius / classification*
Streptococcus salivarius / genetics*
Streptococcus salivarius / isolation & purification

Substances

Bacterial Proteins
salivaricin A