Small protein sequences can induce cellular uptake of complex nanohybrids

Jan-Philip Merkl; Malak Safi; Christian Schmidtke; Fadi Aldeek; Johannes Ostermann; Tatiana Domitrovic; Sebastian Gärtner; John E Johnson; Horst Weller; Hedi Mattoussi

doi:10.3762/bjnano.10.238

Small protein sequences can induce cellular uptake of complex nanohybrids

Beilstein J Nanotechnol. 2019 Dec 12:10:2477-2482. doi: 10.3762/bjnano.10.238. eCollection 2019.

Authors

Jan-Philip Merkl^{1

2

3}, Malak Safi^{1

4}, Christian Schmidtke^{2

3}, Fadi Aldeek^{1

5}, Johannes Ostermann^{2

6}, Tatiana Domitrovic^{7

8}, Sebastian Gärtner^{2

9}, John E Johnson⁸, Horst Weller^{2

3

6}, Hedi Mattoussi¹

Affiliations

¹ Department of Chemistry and Biochemistry, Florida State University, 95 Chieftan Way, Tallahassee, Florida 32306, United States.
² Institute of Physical Chemistry; University of Hamburg, Grindelallee 117, 20146 Hamburg, Germany.
³ The Hamburg Center for Ultrafast Imaging, University of Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany.
⁴ Laboratoire Physique des Solides, UMR 8502, Université de Paris Sud bât 510, 91405 Orsay Cedex, France.
⁵ Altria Center for Research and Technology, 601 E Jackson Street, Richmond, VA, 23219, United States.
⁶ Center for Applied Nanotechnology (CAN) GmbH, Grindelallee 117, 20146 Hamburg, Germany.
⁷ Instituto de Microbiologia Paulo de Goes, Universidade Federal do Rio de Janeiro, 310. Lab I014, 21941-902, Rio de Janeiro, Brazil.
⁸ The Scripps Research Institute, Department of Integrative Structural and Computational Biology, MB31, La Jolla, California 92037, United States.
⁹ Universitätsklinikum Hamburg Eppendorf, 20246, Martinistraße 52, 20251 Hamburg, Germany.

Abstract

In this letter, we report on the ability of functional fusion proteins presenting a lytic gamma peptide, to promote interactions with HeLa cells and delivery of large hybrid nanostructures.

Keywords: bioconjugation; cellular uptake; nanoparticle hybrids; polymer encapsulation; self-assembly.