S-100b protein regulates the activity of skeletal muscle adenylate cyclase in vitro

FEBS Lett. 1988 Nov 21;240(1-2):177-80. doi: 10.1016/0014-5793(88)80363-6.

Abstract

We have investigated the effect of the b isoform of S-100 proteins on adenylate cyclase activity of rat skeletal muscle. S-100b inhibits the adenylate cyclase activity in the presence of Mg2+ (5.0-50 mM), while it activates the same enzyme in the presence of Ca2+ (0.1-1.0 mM) dose-dependently in both cases. S-100b counteracts the stimulatory effect of NaF on adenylate cyclase in the presence of Mg2+ and the inhibitory effect of RMI 12330 A in the presence of Ca2+.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenylyl Cyclase Inhibitors
  • Adenylyl Cyclases / metabolism*
  • Animals
  • Calcium / physiology
  • Enzyme Activation
  • Imines / pharmacology
  • Magnesium / physiology
  • Muscles / enzymology*
  • Rats
  • S100 Proteins / physiology*
  • Sodium Fluoride / pharmacology

Substances

  • Adenylyl Cyclase Inhibitors
  • Imines
  • S100 Proteins
  • RMI 12330A
  • Sodium Fluoride
  • Adenylyl Cyclases
  • Magnesium
  • Calcium