Stereospecific bioactions of 5-hydroxyeicosatetraenoate

FEBS Lett. 1988 Nov 21;240(1-2):163-6. doi: 10.1016/0014-5793(88)80360-0.

Abstract

5(S)-Hydroxyicosatetraenoate stimulates human polymorphonuclear neutrophils (PMNs) to raise their cytosolic calcium. It also potentiates the cells' degranulation responses to platelet-activating factor and diacylglycerols. We synthesized 5(R)-hydroxyicosatetraenoate and found it to be 20-100-fold weaker than the natural isomer in these assays. Thus, the arachidonic acid metabolite activates PMNs by a stereospecific possibly receptor-mediated mechanism.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Calcium / physiology
  • Diglycerides / pharmacology
  • Exocytosis / drug effects
  • Glucuronidase / metabolism
  • Humans
  • Hydroxyeicosatetraenoic Acids / pharmacology*
  • Muramidase / metabolism
  • Neutrophils / drug effects*
  • Platelet Activating Factor / pharmacology
  • Stereoisomerism
  • Tetradecanoylphorbol Acetate / pharmacology

Substances

  • Diglycerides
  • Hydroxyeicosatetraenoic Acids
  • Platelet Activating Factor
  • 1,2-dioctanoylglycerol
  • 5-hydroxy-6,8,11,14-eicosatetraenoic acid
  • Muramidase
  • Glucuronidase
  • Tetradecanoylphorbol Acetate
  • Calcium