Abstract
5(S)-Hydroxyicosatetraenoate stimulates human polymorphonuclear neutrophils (PMNs) to raise their cytosolic calcium. It also potentiates the cells' degranulation responses to platelet-activating factor and diacylglycerols. We synthesized 5(R)-hydroxyicosatetraenoate and found it to be 20-100-fold weaker than the natural isomer in these assays. Thus, the arachidonic acid metabolite activates PMNs by a stereospecific possibly receptor-mediated mechanism.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Calcium / physiology
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Diglycerides / pharmacology
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Exocytosis / drug effects
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Glucuronidase / metabolism
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Humans
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Hydroxyeicosatetraenoic Acids / pharmacology*
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Muramidase / metabolism
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Neutrophils / drug effects*
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Platelet Activating Factor / pharmacology
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Stereoisomerism
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Tetradecanoylphorbol Acetate / pharmacology
Substances
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Diglycerides
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Hydroxyeicosatetraenoic Acids
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Platelet Activating Factor
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1,2-dioctanoylglycerol
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5-hydroxy-6,8,11,14-eicosatetraenoic acid
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Muramidase
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Glucuronidase
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Tetradecanoylphorbol Acetate
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Calcium