Genome Resource for the Huanglongbing Causal Agent ' Candidatus Liberibacter asiaticus' Strain AHCA17 from Citrus Root Tissue in California, USA

Weili Cai; Schyler Nunziata; Stefano Costanzo; Lucita Kumagai; John Rascoe; Michael J Stulberg

doi:10.1094/PDIS-08-19-1735-A

Genome Resource for the Huanglongbing Causal Agent ' Candidatus Liberibacter asiaticus' Strain AHCA17 from Citrus Root Tissue in California, USA

Plant Dis. 2020 Mar;104(3):627-629. doi: 10.1094/PDIS-08-19-1735-A. Epub 2020 Jan 7.

Authors

Weili Cai^{1

2}, Schyler Nunziata¹, Stefano Costanzo¹, Lucita Kumagai³, John Rascoe¹, Michael J Stulberg¹

Affiliations

¹ United States Department of Agriculture-Animal and Plant Health Inspection Service, Plant Protection and Quarantine, Science and Technology, Beltsville, MD.
² Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC.
³ Plant Pests Diagnostics Branch, California Department of Food and Agriculture, Sacramento, CA.

PMID: 31910115
DOI: 10.1094/PDIS-08-19-1735-A

Abstract

'Candidatus Liberibacter asiaticus' is the unculturable causative agent of citrus huanglongbing disease. Here, we report the first citrus root metagenome sequence containing the draft genome of 'Ca. L. asiaticus' strain AHCA17, obtained from a pummelo tree in California. The assembled genome was 1.2 Mbp and resulted in 37 contigs (N₅₀ = 158.7 kbp) containing 1,057 predicted open reading frames and 45 RNA-coding genes. This draft genome will provide a valuable resource in further study of 'Ca. L. asiaticus' genome diversity and pathogen epidemiology.

Keywords: citrus; metagenomics; prokaryotes.

MeSH terms

California
Citrus*
Plant Diseases
Rhizobiaceae*