RNA sequencing data of mouse 2-cell embryos treated with DMSO

Min-Hee Kang; Seong-Yeob You; Kwonho Hong; Jin-Hoi Kim

doi:10.1016/j.dib.2019.105025

RNA sequencing data of mouse 2-cell embryos treated with DMSO

Data Brief. 2019 Dec 19:28:105025. doi: 10.1016/j.dib.2019.105025. eCollection 2020 Feb.

Authors

Min-Hee Kang¹, Seong-Yeob You¹, Kwonho Hong¹, Jin-Hoi Kim¹

Affiliation

¹ Department of Stem Cell and Regenerative Biotechnology, Humanized Pig Research Center (SRC), Konkuk University, Seoul, Republic of Korea.

Abstract

To understand the effect of DMSO in preimplantation embryos, we have treated mouse 1 cell zygotes with DMSO and found that DMSO treatment caused 2 or 4 cell embryonic arrest and altered the acetylation levels of mouse preimplantation embryos To illustrate the mechanism of DMSO in mouse preimplantation embryos, fertilized zygotes have been treated with 2% of DMSO and then performed RNA-seq analyses. Differentially expressed genes were identified using DESeq2 after adjustment for false discovery rate (FDR q value < 0.05). Gene Set Enrichment Analysis (GSEA) was also performed to identify biological pathways significantly modulated by DMSO. Raw and processed RNA-seq data were deposited and made publicly available on the Gene Expression Omnibus (GEO; GSE124598). The data presented in this article are related to the research paper entitled "DMSO impairs the transcriptional program for maternal-to-embryonic transition by altering histone acetylation", available in Biomaterials [1].

Keywords: Acetylation; Dimethyl sulfoxide; Epigenetics; Preimplantation embryo; RNA sequencing.