Data on Tougu Xiaotong capsules may inhibit p38 MAPK pathway-mediated inflammation in vitro

Xihai Li; Zhenli Zhang; Wenna Liang; Jianwei Zeng; Xiang Shao; Limei Xu; Liangliang Jia; Xiaojuan He; Hui Li; Chunsong Zheng; Hongzhi Ye; Tetsuya Asakawa

doi:10.1016/j.dib.2019.105023

Data on Tougu Xiaotong capsules may inhibit p38 MAPK pathway-mediated inflammation in vitro

Data Brief. 2019 Dec 19:28:105023. doi: 10.1016/j.dib.2019.105023. eCollection 2020 Feb.

Authors

Xihai Li^{1

2}, Zhenli Zhang³, Wenna Liang⁴, Jianwei Zeng¹, Xiang Shao¹, Limei Xu¹, Liangliang Jia¹, Xiaojuan He¹, Hui Li¹, Chunsong Zheng¹, Hongzhi Ye^{1

2}, Tetsuya Asakawa^{4

5}

Affiliations

¹ Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, PR China.
² Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fuzhou 350122, PR China.
³ SIPO Patent Examination (Beijing) Center, Beijing 100160, PR China.
⁴ Research Base of Traditional Chinese Medicine Syndrome, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China.
⁵ Department of Neurosurgery, Hamamatsu University School of Medicine, Handayama, 1-20-1, Higashi-ku, Hamamatsu-city, Shizuoka 431-3192, Japan.

Abstract

The Tougu Xiaotong capsule (TXC) is a traditional herbal compound used to treat osteoarthritis (OA) in China. We performed fingerprint analysis with HPLC for the quality control of TXC. Its composition was identified by the comparison of the spectrogram and chromatographic peak of retention time with a reference substance. TXC was found to contain paeoniflorin, isofraxidin, ferulic acid, and rosmarinic acid. The chondrocytes were identified by immunohistochemical staining using collagen II. Chondrocytes that were positive for collagen II were stained brown in the cytoplasm. The toll-like receptor 4 (TLR4) was expressed on the chondrocyte membrane, which was observed using immunofluorescence microscopy. The nuclei were stained blue by 4',6-diamidino-2-phenylindole (DAPI) and TLR4 was stained green. These were observed using laser scanning confocal microscopy. The successful establishment of LPS-exposed chondrocytes was confirmed using enzyme-linked immunosorbent assay (ELISA). Lipopolysaccharide (LPS) administration significantly reduced the levels of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α), and a maximum effect was observed at 8 h. We believe that these methods will be useful in future investigations of OA. This data article is related to the research article "Tougu Xiaotong capsules may inhibit p38 MAPK pathway-mediated inflammation: In vivo and in vitro verification" [1].

Keywords: Chondrocyte; Inflammation; Osteoarthritis; Toll like receptor 4; Tougu Xiaotong capsule.