Interleukin 10 and Transforming Growth Factor Beta Polymorphisms as Risk Factors for Kawasaki Disease: A Case-Control Study and Meta-Analysis

Farzaneh Rahmani; Vahid Ziaee; Raheleh Assari; Maryam Sadr; Arezou Rezaei; Zeinab Sadr; Seyed Reza Raeeskarami; Mohammad Hassan Moradinejad; Yahya Aghighi; Nima Rezaei

Interleukin 10 and Transforming Growth Factor Beta Polymorphisms as Risk Factors for Kawasaki Disease: A Case-Control Study and Meta-Analysis

Avicenna J Med Biotechnol. 2019 Oct-Dec;11(4):325-333.

Authors

Farzaneh Rahmani^{1

2}, Vahid Ziaee^{3

4}, Raheleh Assari^{3

4}, Maryam Sadr⁵, Arezou Rezaei¹, Zeinab Sadr⁵, Seyed Reza Raeeskarami⁶, Mohammad Hassan Moradinejad⁴, Yahya Aghighi⁷, Nima Rezaei^{1

8

9}

Affiliations

¹ Research Center for Immunodeficiencies, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran.
² NeuroImaging Network (NIN), Universal Scientific Education and Research Network (USERN), Tehran, Iran.
³ Pediatric Rheumatology Research Group, Rheumatology Research Center, Tehran University of Medical Sciences, Tehran, Iran.
⁴ Department of Pediatrics, Pediatrics Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran.
⁵ Molecular Immunology Research Center, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
⁶ Department of Pediatrics, Vali-e-Asr Hospital, Tehran University of Medical Sciences, Tehran, Iran.
⁷ Department of Pediatrics, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran.
⁸ Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
⁹ Network of Immunity in Infection, Malignancy and Autoimmunity (NIIMA), Universal Scientific Education and Research Network (USERN), Boston, MA, USA.

PMID: 31908741
PMCID: PMC6925396

Abstract

Background: Alteration in serum expression of Transforming Growth Factor-beta (TGF-β) and IL-10 have been suggested to play a role in the pathogenesis of Kawasaki Disease (KD). Inconsistent reports exist on the association of IL-10 polymorphisms with KD susceptibility and Coronary Artery Aneurysms (CAA).

Methods: A number of 110 paediatric patients with KD and 140 healthy individuals were recruited to investigate the frequency of Single Nucleotide Polymorphisms (SNPs) of TGF-β C/T at codon 10 (rs1982073), C/G at codon 25 (rs1800471) and IL-10 A/G at -1082 (rs1800896), C/T at -819 (rs1800871) and A/C at -592 (rs1800872) and their respective genotype and haplotypes. A comprehensive search was performed in MEDLINE and SCOPUS using the keywords of interleukin 10, transforming growth factor beta, and Kawasaki disease. Moreover, previous studies investigating the TGF-β and IL-10 polymorphisms in KD were evaluated. Review Manager Version 5.1 Software was used to perform meta-analysis.

Results: There was no significant association between allelic or genotypic variants in the mentioned polymorphisms in TGF-β or IL-10 with KD or CAA. The only significant haplotypic variant was TC variant at codon 10, and 25 of TGF-β polymorphisms were associated with higher risk of KD. Meta-analysis of a total number of 770 patients vs. 1471 healthy controls showed no difference in the frequency of any of the IL-10 genetic variants in KD patients, regardless of the presence of CAA.

Conclusion: Polymorphisms of TGF-β or IL-10 are not associated with additional risk for KD in Iranian population. IL-10 polymorphisms at -1082, -819 and -592 positions are not associated with KD, nor do they predict coronary artery aneurysm formation.

Keywords: Cytokine; Interleukin-10; Kawasaki disease; Single nucleotide polymorphisms; Transforming growth factor-beta.