Simian immunodeficiency virus infection and flow cytometric characterization of Japanese macaque (Macaca fuscata) hematopoietic cells

Tetsuo Tsukamoto; Katsuki Nakamura; Seiji Okada

doi:10.1111/jmp.12460

Simian immunodeficiency virus infection and flow cytometric characterization of Japanese macaque (Macaca fuscata) hematopoietic cells

J Med Primatol. 2020 Apr;49(2):116-120. doi: 10.1111/jmp.12460. Epub 2020 Jan 6.

Authors

Tetsuo Tsukamoto^{1

2}, Katsuki Nakamura³, Seiji Okada¹

Affiliations

¹ Division of Hematopoiesis, Center for AIDS Research, Kumamoto University, Kumamoto, Japan.
² Department of Immunology, Kindai University Faculty of Medicine, Osaka, Japan.
³ Primate Research Institute, Kyoto University, Inuyama, Japan.

PMID: 31907946
DOI: 10.1111/jmp.12460

Abstract

We characterized Japanese macaque (Macaca fuscata) hematopoietic cells using flow cytometry and identified 28 cross-reactive anti-human antibody clones. Furthermore, productive infection of peripheral T lymphocytes with simian immunodeficiency virus (SIV) in vitro was confirmed by intracellular SIV p27 staining. This study could facilitate using Japanese macaques as models for human hematological and immunological disorders and infectious diseases.

Keywords: CD4+ T lymphocytes; acquired immunodeficiency syndrome; hematopoietic progenitor cells; monkey AIDS models; simian immunodeficiency virus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Disease Models, Animal
Female
Flow Cytometry / veterinary*
Macaca fuscata*
Male
Simian Acquired Immunodeficiency Syndrome / virology*
Simian Immunodeficiency Virus / isolation & purification*
T-Lymphocytes / virology*