Objective: explore the expression of miR-155-5p in Wilms tumor and its effect in regulating the proliferation, migration and apoptosis of Wilms tumor cells.
Methods: Specimens of tumor tissues and paired adjacent tissues were obtained from 40 patients with Wilms tumor for detection of the expression levels of miR-155-5p using RT-qPCR. Wilms tumor cell line G401 was transfected with miR-155-5p mimics and miR-155-5p inhibitor to induce miR-155-5p over-expression and its inhibition, respectively, and the changes in the cell proliferation, migration and apoptosis were assessed using cell counting kit-8 (CCK-8), wound healing assay and fl ow cytometry.
Results: RT-qPCR showed that the expression of miR-155-5p decreased significantly in Wilms tumor tissues as compared with normal kidney tissues and was significantly associated with TNM stage (P < 0.05). In G401 cells, over-expression of miR-155-5p significantly inhibited the cell proliferation and migration and promoted cell apoptosis (P < 0.05), and down-regulation of miR-155-5p obviously enhanced the proliferation and migration and suppressed apoptosis of the cells (P < 0.05).
Conclusions: miR-155-5p is down-regulated in Wilms tumor and its expression level is correlated with TNM stage. miR-155-5p participates in the progression of Wilms tumor by inhibiting the proliferation and migration and promoting apoptosis of the tumor cells, and may serve as a novel biomarker for diagnosis, therapy and prognostic evaluation of Wilms tumor.
目的: 探讨miR-155-5p在肾母细胞瘤中的表达及其对肾母细胞瘤细胞增殖、迁移及凋亡能力的影响。
方法: 收集40例肾母细胞瘤患者的肿瘤组织及其相对应的瘤旁组织，瘤旁组织作为正常对照组，应用RTqPCR检测肾母细胞瘤组织和正常瘤旁肾脏组织中miR-155-5p的表达水平；在肾母细胞瘤细胞株G401中，运用脂质体转染技术过表达及抑制miR-155-5p；采用CCK-8实验检测肾母细胞瘤细胞增殖能力的变化；划痕实验检测肾母细胞瘤细胞迁移能力的变化；流式细胞术检测肾母细胞瘤细胞凋亡能力的变化。
结果: RTqPCR结果显示肾母细胞瘤肿瘤组织中miR-155-5p的表达水平显著低于正常肾脏组织，且miR-155- 5p的表达与肾母细胞瘤的临床分期呈负相关，差异有统计学意义（P < 0.05）；在人肾母细胞瘤细胞G401中上调miR-155-5p表达能够显著抑制肿瘤细胞的增殖和迁移，促进凋亡（P < 0.05）；下调miR-155-5p表达能够显著促进肿瘤细胞的增殖和迁移，抑制凋亡（P < 0.05）。
结论: miR-155-5p在肾母细胞瘤患者肿瘤组织中表达显著下调，且其表达量与临床分期呈负相关；miR-155-5p可抑制肾母细胞瘤细胞增殖和迁移，促进细胞凋亡；miR-155-5p有望成为肾母细胞瘤诊断、治疗和预后评估的一个新的潜在标志物。
Keywords: Wilms tumor; apoptosis; miR-155-5p; migration; proliferation.