Bv8-Like Toxin from the Frog Venom of Amolops jingdongensis Promotes Wound Healing via the Interleukin-1 Signaling Pathway

Jiajia Chang; Xiaoqin He; Jingmei Hu; Peter Muiruri Kamau; Ren Lai; Dingqi Rao; Lei Luo

doi:10.3390/toxins12010015

Bv8-Like Toxin from the Frog Venom of Amolops jingdongensis Promotes Wound Healing via the Interleukin-1 Signaling Pathway

Toxins (Basel). 2019 Dec 29;12(1):15. doi: 10.3390/toxins12010015.

Authors

Jiajia Chang^{1

2}, Xiaoqin He^{2

3

4}, Jingmei Hu^{2

3}, Peter Muiruri Kamau^{2

5

6}, Ren Lai^{2

3

6

7

8

9}, Dingqi Rao¹⁰, Lei Luo²

Affiliations

¹ College of Life Sciences, Anhui Normal University, Wuhu 241000, China.
² Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences/Key Laboratory of Bioactive Peptides of Yunnan Province, National & Local Joint Engineering Laboratory of Natural Peptides, Kunming Institute of Zoology, Kunming 650223, China.
³ College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
⁴ Key Laboratory of National Health and Family Planning Commission on Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi 214064, China.
⁵ University of Chinese Academy of Sciences, Beijing 100049, China.
⁶ Sino-African Joint Research Center, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China.
⁷ KIZ-CUHK Joint Laboratory of Bioresources and Molecular Research in Common Diseases, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China.
⁸ Institute for Drug Discovery and Development, Chinese Academy of Sciences, Shanghai 201203, China.
⁹ Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan 430207, China.
¹⁰ State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China.

Abstract

Prokineticins are highly conserved small peptides family expressed in all vertebrates, which contain a wide spectrum of functions. In this study, a prokineticin homolog (Bv8-AJ) isolated from the venom of frog Amolops jingdongensis was fully characterized. Bv8-AJ accelerated full-thickness wounds healing of mice model by promoting the initiation and the termination of inflammatory phase. Moreover, Bv8-AJ exerted strong proliferative effect on fibroblasts and keratinocytes isolated from newborn mice by activating interleukin (IL)-1 production. Our findings indicate that Bv8 is a potent wound healing regulator and may reveal the mechanism of rapid wound-healing in amphibian skins.

Keywords: Amolops jingdongensis; Bv8; Interleukin-1; fibroblasts; keratinocytes; wound healing.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amphibian Venoms / pharmacology*
Animals
Animals, Newborn
Anti-Inflammatory Agents / pharmacology
Cell Proliferation / drug effects
Female
Fibroblasts / drug effects
Interleukin-1* / biosynthesis
Keratinocytes / drug effects
MAP Kinase Signaling System / drug effects
Male
Mice
RAW 264.7 Cells
Ranidae
Rats
Signal Transduction / drug effects*
Wound Healing / drug effects*

Substances

Amphibian Venoms
Anti-Inflammatory Agents
Interleukin-1