Nonuniform azimuthal distribution of N2+ fluorescence emitted from the femtosecond laser filament in air was discovered. The fluorescence is stronger when the detector is placed perpendicular or parallel to the laser polarization. The experimental results have been confirmed by the theoretical calculation that the azimuthal distribution of fluorescence is reproduced by the convolution of the transition of the dipole and the molecular alignment in the strong laser field. The results would provide new insight into laser-molecule interactions during filamentation, which are important in practice for remote sensing using filamentation.