Identification of the wild and cultivated hosts of wheat dwarf virus and oat dwarf virus in Iran

Najmeh Pouramini; Jahangir Heydarnejad; Hossain Massumi; Arvind Varsani

doi:10.1007/s13337-019-00557-y

Identification of the wild and cultivated hosts of wheat dwarf virus and oat dwarf virus in Iran

Virusdisease. 2019 Dec;30(4):545-550. doi: 10.1007/s13337-019-00557-y. Epub 2019 Nov 28.

Authors

Najmeh Pouramini¹, Jahangir Heydarnejad¹, Hossain Massumi¹, Arvind Varsani^{2

3}

Affiliations

¹ 1Department of Plant Protection, College of Agriculture, Shahid Bahonar University of Kerman, Kerman, 7616914111 Iran.
² 2The Biodesign Center of Fundamental and Applied Microbiomics, School of Life Sciences, Center for Evolution and Medicine, Arizona State University, 1001 S. McAllister Ave, Tempe, AZ 85287-5001 USA.
³ 3Structural Biology Research Unit, Department of Integrative Biomedical Sciences, University of Cape Town, Observatory, Cape Town, South Africa.

Abstract

In the last decade two mastreviruses, Wheat dwarf virus (WDV) and Oat dwarf virus (ODV) have been reported from cereal farms in Iran. In a survey, wild and cultivated hosts of these mastreviruses were studied during 2015 to 2017. Symptomatic small grain cereal samples and weed species were collected and assayed for WDV and/or ODV infection by PCR. While WDV which was detected in 139/284 (49%) of total symptomatic samples, low incidence (2%) was recorded for ODV which was detected only in slender wild oat (Avena barbata Pott ex Link) and red brome (Bromus rubens L.). In agroinfection studies, the clone of ODV infected common oat (A. sativa) and slender wild oat (A. barbata) with the low efficiency and did not infect wheat or barley. ODV was transmitted by the leafhopper Psammotettix alienus, from agroinfected common oat to healthy seedlings. The results show that, in contrast to WDV, ODV has a low incidence and a narrow host range in gramineous plants.

Keywords: Agroinfection; Geminivirus; Mastrevirus; Oat dwarf virus; Psammotettix alienus; Wheat dwarf virus.