Anti-degranulation response of herbal formula in RBL-2H3 cells

Jing Hu; Yujuan Chen; Jiajing Zhu; Mingyan Gao; Jiani Li; Zhengxun Song; Hongmei Xu; Zuobin Wang

doi:10.1016/j.micron.2019.102819

Anti-degranulation response of herbal formula in RBL-2H3 cells

Micron. 2020 Mar:130:102819. doi: 10.1016/j.micron.2019.102819. Epub 2019 Dec 28.

Authors

Jing Hu¹, Yujuan Chen², Jiajing Zhu³, Mingyan Gao¹, Jiani Li¹, Zhengxun Song¹, Hongmei Xu¹, Zuobin Wang⁴

Affiliations

¹ Ministry of Education Key Laboratory for Cross-Scale Micro and Nano Manufacturing, Changchun University of Science and Technology, Changchun 130022, China; International Research Centre for Nano Handling and Manufacturing of China, Changchun University of Science and Technology, Changchun 130022, China.
² Ministry of Education Key Laboratory for Cross-Scale Micro and Nano Manufacturing, Changchun University of Science and Technology, Changchun 130022, China; International Research Centre for Nano Handling and Manufacturing of China, Changchun University of Science and Technology, Changchun 130022, China; School of Life Sciences, Changchun University of Science and Technology, Changchun 130022, China. Electronic address: chenyujuan@cust.edu.cn.
³ School of Engineering, University of Warwick, Coventry CV4 7AL, UK.
⁴ Ministry of Education Key Laboratory for Cross-Scale Micro and Nano Manufacturing, Changchun University of Science and Technology, Changchun 130022, China; International Research Centre for Nano Handling and Manufacturing of China, Changchun University of Science and Technology, Changchun 130022, China; JR3CN & IRAC, University of Bedfordshire, Luton LU1 3JU, UK. Electronic address: wangz@cust.edu.cn.

PMID: 31896517
DOI: 10.1016/j.micron.2019.102819

Abstract

Allergic diseases not only bring serious economic burden to the patients, but also consume a lot of substantial resources of social medical systems. Thus, the prevention and treatment of allergic diseases are imperative. In this study, the anti-degranulation activity of herbal formula was evaluated using the rat basophil leukemia cells (RBL-2H3) as in vitro model. The morphological and biophysical properties of RBL-2H3 cells before and after treatment with herbal formula were also determined. Notably, the herbal formula exhibits clearly inhibited degranulation by RBL-2H3 cells in a concentration-dependent manner without cytotoxic effect. Therefore, this herbal formula can be used as an alternative and promising therapeutic agent to ameliorate allergic diseases.

Keywords: Allergic diseases; Anti-degranulation; Herbal formula; RBL-2H3 cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Basophils / drug effects*
Basophils / ultrastructure
Cell Degranulation / drug effects*
Cell Line, Tumor
Cell Survival / drug effects*
Drugs, Chinese Herbal / pharmacology*
Microscopy, Atomic Force
Microscopy, Electron, Scanning
Rats

Substances

Drugs, Chinese Herbal