Method for Characterization of Root Traits in Chickpea Germplasm for Legume Genomics and Breeding

Methods Mol Biol. 2020:2107:269-275. doi: 10.1007/978-1-0716-0235-5_14.

Abstract

A semi-hydroponic phenotyping platform was constructed using inexpensive and easily obtained materials for characterizing root trait variability in a large set of chickpea (Cicer arietinum) germplasm. The system was designed to accommodate a large number of plants in a small area allowing relatively deeper root development, and thus serves as a high-throughput phenotyping tool for studying root dynamic growth. The root trait quantitative platform could provide accurate phenotyping data for parameterizing root models and for genome-wide association analyses or mapping studies of quantitative trait loci.

Keywords: Chickpea; Phenotyping; Root traits; Semi-hydroponic system.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromosome Mapping
  • Cicer / genetics
  • Cicer / growth & development*
  • Genome-Wide Association Study
  • High-Throughput Screening Assays
  • Phenotype
  • Plant Breeding
  • Plant Roots / genetics
  • Plant Roots / growth & development
  • Quantitative Trait Loci*
  • Seeds / genetics
  • Seeds / growth & development*