Multiparameter toxicity screening on a chip: Effects of UV radiation and titanium dioxide nanoparticles on HaCaT cells

Scott McCormick; Louise E Smith; Amy M Holmes; Ziqiu Tong; Enzo Lombi; Nicolas H Voelcker; Craig Priest

doi:10.1063/1.5113729

Multiparameter toxicity screening on a chip: Effects of UV radiation and titanium dioxide nanoparticles on HaCaT cells

Biomicrofluidics. 2019 Aug 27;13(4):044112. doi: 10.1063/1.5113729. eCollection 2019 Jul.

Authors

Scott McCormick, Louise E Smith¹, Amy M Holmes², Ziqiu Tong³, Enzo Lombi¹, Nicolas H Voelcker, Craig Priest¹

Affiliations

¹ Future Industries Institute, University of South Australia, Mawson Lakes Campus, Mawson Lakes Boulevard, Mawson Lakes, SA 5095, Australia.
² School of Pharmacy and Medical Sciences, University of South Australia, Adelaide, SA 5000, Australia.
³ Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, VIC 3052, Australia.

Abstract

Microfluidic screening is gaining attention as an efficient method for evaluating nanomaterial toxicity. Here, we consider a multiparameter treatment where nanomaterials interact with cells in the presence of a secondary exposure (UV radiation). The microfluidic device contains channels that permit immobilization of HaCaT cells (human skin cell line), delivery of titanium dioxide nanoparticles (TNPs), and exposure to a known dose of UV radiation. The effect of single-parameter exposures (UV or TNP) was first studied as a benchmark, and then multiparameter toxicity (UV and TNP) at different concentrations was explored. The results demonstrate a concentration-dependent protective effect of TNP when exposed to UV irradiation.