Capacitance electrochemical biosensor based on silicon nitride transducer for TNF-α cytokine detection in artificial human saliva: Heart failure (HF)

Talanta. 2020 Mar 1:209:120501. doi: 10.1016/j.talanta.2019.120501. Epub 2019 Oct 23.

Abstract

In the present study, we have developed a capacitance electrochemical biosensor based on silicon nitride substrate (Si3N4/SiO2/Si[P]/Al) for Tumour Necrosis Factor Alpha (TNF-α) cytokines detection. Micro-contact printing, Fluorescence microscopy characterization and contact angle measurement (CAM) were carried out during the bio-functionalization of the biosensor surface. Mott-Schottky analyses were applied for TNF-α detection within the range of 1 pg/mL to 30 pg/mL in which the immunosensor has exhibited a good linearity, a sensitivity of 4 mV.pM-1 and 4.4 mV.pM-1 in PBS and artificial saliva (AS) respectively. While the LOD was found at 0.38 pg/mL and 1 pg/mL in PBS and AS respectively. The developed immunosensor has also demonstrated a high and good selectivity for TNF-α detection in human AS when compared to other interferences like Cortisol and Interleukin-10. The performances of the developed biosensor are very promising for biomedical application to predict the first sign of inflammation.

Keywords: Electrochemical biosensor; Human artificial saliva; Mott-Schottky; Silicon nitride transducer; Tumour Necrosis Factor.

MeSH terms

  • Antibodies, Immobilized / immunology
  • Antibodies, Monoclonal / immunology
  • Biosensing Techniques / methods*
  • Electric Capacitance
  • Electrochemical Techniques / instrumentation
  • Electrochemical Techniques / methods*
  • Electrodes
  • Fluorescent Dyes / chemistry
  • Humans
  • Immunoassay / methods
  • Limit of Detection
  • Rhodamines / chemistry
  • Saliva, Artificial / chemistry*
  • Silicon Compounds / chemistry*
  • Silicon Dioxide / chemistry
  • Transducers*
  • Tumor Necrosis Factor-alpha / analysis*
  • Tumor Necrosis Factor-alpha / immunology

Substances

  • Antibodies, Immobilized
  • Antibodies, Monoclonal
  • Fluorescent Dyes
  • Rhodamines
  • Saliva, Artificial
  • Silicon Compounds
  • TNF protein, human
  • Tumor Necrosis Factor-alpha
  • Silicon Dioxide
  • silicon nitride