Determination of prostate cancer marker Zn2+ with a highly selective surface-enhanced Raman scattering probe on liquid-liquid self-assembled Au nanoarrays

Abstract

As the concentration of Zn²⁺ in patients with prostate cancer is much less than that in healthy persons, Zn²⁺ concentration can be used as a marker to expediently screen prostate cancer. In this study, a sensitive and highly selective surface-enhanced Raman scattering (SERS) method to detect Zn²⁺ concentration in human prostatic fluids by utilizing water-insoluble 2-carboxyl-2'-hydroxyl-5'-sulfoformazylbenze (Zincon) as a SERS probe based on self-assembled Au nanoarrays at a liquid-liquid interface between n-hexane and Au colloids was proposed. Zincon showed remarkably different SERS bands before and after coordinating Zn²⁺ in the controlled conditions (70 μL of ethanol, 500 μL of n-hexane, pH value of 7.1 and 10 s of vortex mixing time), which can be used in quantifying Zn²⁺ with characteristic peaks. The proposed SERS method presented a good linear relationship ranging from 0.5 to 10 μmol/L and a satisfactory detection limit of 0.1 μmol/L as well as low interference with other metal ions. Moreover, the detection results are close to those of the conventional standard atomic absorption spectroscopy (AAS) method.

Keywords: Au nanoarray; Liquid-liquid self-assembly; Prostate cancer; SERS; Zincon; Zn(2+).