Anticancer activity and metabolite profiling data of Penicillium janthinellum KTMT5

Kudzanai Ian Tapfuma; Tendani Edith Sebola; Nkemdinma Uche-Okereafor; Jody Koopman; Raeesa Hussan; Maya Mellisa Makatini; Lukhanyo Mekuto; Vuyo Mavumengwana

doi:10.1016/j.dib.2019.104959

Anticancer activity and metabolite profiling data of Penicillium janthinellum KTMT5

Data Brief. 2019 Dec 7:28:104959. doi: 10.1016/j.dib.2019.104959. eCollection 2020 Feb.

Authors

Kudzanai Ian Tapfuma¹, Tendani Edith Sebola², Nkemdinma Uche-Okereafor², Jody Koopman¹, Raeesa Hussan¹, Maya Mellisa Makatini³, Lukhanyo Mekuto⁴, Vuyo Mavumengwana¹

Affiliations

¹ DST/NRF Centre of Excellence for Biomedical Tuberculosis Research and SAMRC Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Department of Biomedical Sciences, Stellenbosch University, PO Box 19063, Tygerberg, 7505, Cape Town, South Africa.
² Department of Biotechnology and Food Technology, Faculty of Science, University of Johannesburg, PO Box 17011, Doornfontein, Johannesburg, 2028, South Africa.
³ Molecular Sciences Institute, School of Chemistry, University of the Witwatersrand, PO Box Wits, 2050, Johannesburg, South Africa.
⁴ Department of Chemical Engineering, Faculty of Engineering and the Built Environment, University of Johannesburg, PO Box 17011, Doornfontein, Johannesburg, 2028, South Africa.

Abstract

Fungi are ubiquitous, they proliferate even in environments with toxic pollutants that are otherwise harmful to other eukaryotes. This article presents data of fungi which were isolated from gold mine tailings and identified by DNA sequencing of their inter transcribed spacer regions 1 and 2. Five fungal isolates were identified, among which the crude extract of Penicillium janthinellum KTMT5 was investigated for anticancer activity on A549 (lung carcinoma) and UMG87 (glioblastoma) cell lines. Untargeted metabolite profiling of the crude extract of P. janthinellum KTMT5 was performed using liquid chromatography quadrupole time of flight tandem mass spectrometry (LC-QTOF-MS/MS) and a molecular network generated using the online workflow on the Global Natural Product Social molecular networking (GNPS) website. DNA sequencing showed that all fungal isolates belonged to phylum Ascomycota with the genus Penicillium representing 75% of the fungal isolates. P. janthinellum KTMT5 which was selected for further experiments showed significant anticancer activity against UMG87 cells with a calculated IC₅₀ value of 44.23 μg/mL in the MTS assay, while the real time xCELLigence assay showed dose-dependent anticancer activity at 50 and 100 μg/mL. Metabolite profiling revealed the presence of several known metabolites in the crude extract of P. janthinellum KTMT5 and molecular networking showed the relationships among these metabolites.

Keywords: Anticancer; LC-QTOF-MS/MS; Metabolite profiling; Mine tailings; Penicillium janthinellum.