Adequate taylor couette flow-mediated shear stress is useful for dissociating human iPS cell-derived cell aggregates

Katsuhisa Matsuura; Masanori Wada; Katsuhisa Sakaguchi; Yuki Matsuhashi; Tatsuya Shimizu

doi:10.1016/j.reth.2019.04.006

Adequate taylor couette flow-mediated shear stress is useful for dissociating human iPS cell-derived cell aggregates

Regen Ther. 2019 Apr 25:12:6-13. doi: 10.1016/j.reth.2019.04.006. eCollection 2019 Dec 15.

Authors

Katsuhisa Matsuura^{1

2}, Masanori Wada³, Katsuhisa Sakaguchi⁴, Yuki Matsuhashi⁵, Tatsuya Shimizu¹

Affiliations

¹ Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku, Tokyo, 162-8666, Japan.
² Department of Cardiology, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku, Tokyo, 162-8666, Japan.
³ ABLE Corporation, 5-9 Nishigoken-cho, Shinjuku, Tokyo, 162-0812, Japan.
⁴ School of Creative Science and Engineering, TWIns, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan.
⁵ Graduate School of Advanced Science and Engineering, TWIns, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan.

Abstract

Pluripotent stem cell including induced pluripotent stem cells (iPSC) are promising cell sources for regenerative medicine and for three-dimensional suspension culture technologies which may enable the generation of robust numbers of desired cells through cell aggregation. Although manual procedure is widely used for dissociating cell aggregates, the development of non-manual procedures using devices will contribute to efficient cell manufacturing. In the present study, we developed novel cell aggregate dissociation devices with a rotating cylinder inside based on taylor couette flow-mediated shear stress. The shear stress can be increased according to an increase in the size of the rotating cylinder inside the devices and the rotation rate. Adequate device size and suitable rotation rate efficiently dissociated cell aggregates after the undifferentiated expansion and the cardiac differentiation of human iPSC. These finding suggest that non-manual device procedure might be useful for harvesting single cells from human iPSC-derived cell aggregates.

Keywords: 3D suspension culture; Cell aggregate dissociation device; Taylor couette flow; iPS cell.