The effect of low level laser irradiation on oxidative stress, muscle damage and function following neuromuscular electrical stimulation. A double blind, randomised, crossover trial

Ewa Jówko; Maciej Płaszewski; Maciej Cieśliński; Tomasz Sacewicz; Igor Cieśliński; Marta Jarocka

doi:10.1186/s13102-019-0147-3

The effect of low level laser irradiation on oxidative stress, muscle damage and function following neuromuscular electrical stimulation. A double blind, randomised, crossover trial

BMC Sports Sci Med Rehabil. 2019 Dec 27:11:38. doi: 10.1186/s13102-019-0147-3. eCollection 2019.

Authors

Ewa Jówko¹, Maciej Płaszewski², Maciej Cieśliński², Tomasz Sacewicz¹, Igor Cieśliński², Marta Jarocka²

Affiliations

¹ 1Józef Piłsudski University of Physical Education in Warsaw; Faculty of Physical Education and Health in Biała Podlaska, Chair of Natural Sciences, Akademicka 2, 21-500 Biała Podlaska, Poland.
² 2Józef Piłsudski University of Physical Education in Warsaw; Faculty of Physical Education and Health in Biała Podlaska, Chair of Rehabilitation, Biała Podlaska, Poland.

Abstract

Background: Low level laser therapy (LLLT) is among novel methods for preventing and treating muscle damage and soreness induced by volitional exercise, but little is known about using LLLT before neuromuscular electrical stimulation. The aim of this first randomised, double blind, crossover trial addressing this issue was to evaluate effects of LLLT on muscle damage and oxidative stress, as well as recovery of muscle function after a single session of isometric neuromuscular electrical stimulation(NMES).

Methods: Twenty four moderately active, healthy men aged 21-22 years received 45 electrically evoked tetanic, isometric contractions of the quadriceps femoris, preceded by LLLT or sham-LLLT. Maximal isometric voluntary muscle torques, perceived soreness, and blood samples were analysed from baseline to 96 h post intervention. We measured plasma markers of muscle damage (the activity of creatine kinase), and inflammation (C-reactive protein), and evaluated redox state parameters.

Results: NMES-evoked contractions induced oxidative stress, demonstrated by an increase in lipid peroxidation and impairments in enzymatic antioxidant system. LLLT irradiations had a protective effect on NMES-induced decrease in enzymatic antioxidant defence and shortened the duration of inflammation. This effect of irradiations on redox state and inflammation did not affect lipid peroxidation, muscle damage, and muscle torque.

Conclusions: LLLT may protect from impairments in enzymatic antioxidant system and may shorten inflammation induced by a single NMES session in moderately active, healthy men. However, the effects of LLLT on redox state and inflammatory processes do not seem to affect muscle damage and recovery of muscle function after NMES.

Trial registration: The study was retrospectively registered in the Australian New Zealand Clinical Trials Registry (ANZCTR); The trial registration number: ACTRN12619000678190; date of registration: 6 May 2019.

Keywords: Biochemistry; DOMS; Evoked contractions; Exercise-induced muscle damage; Knee extensors; Photobiomodulation.