Efficient polysaccharides from Crinum asiaticum L.'s structural characterization and anti-tumor effect

Miao Yu; Yingxiang Chen; Yingjie Liu; Miao Yu; Ying Xu; Bing Wang

doi:10.1016/j.sjbs.2019.09.017

Efficient polysaccharides from Crinum asiaticum L.'s structural characterization and anti-tumor effect

Saudi J Biol Sci. 2019 Dec;26(8):2085-2090. doi: 10.1016/j.sjbs.2019.09.017. Epub 2019 Sep 14.

Authors

Miao Yu^{1

2}, Yingxiang Chen¹, Yingjie Liu^{1

2}, Miao Yu¹, Ying Xu¹, Bing Wang³

Affiliations

¹ Research Center on Life Science and Environmental Science, Harbin University of Commerce, Harbin 150076, China.
² Postdoctoral Scientific Research Workstation, Harbin University of Commerce, Harbin 150076, China.
³ School of Food Engineering, Harbin University of Commerce, Harbin 150076, China.

Abstract

In this study, an efficient polysaccharide, named CAL-n (Crinum asiaticum L.-n) was isolated and purified from Crinum asiaticum L for the first time, Mw(molecular weight) of 730,000 Da. CAL-n comprised Rha(rhamnose), Sor(sorbose), Gal(galactose) and Glu(glucosein) the molar ratio of 1:61.6:1.66:4.74. The chemical structure of CAL-n was studied by Infrared spectrum and GC-MS(Gas Chromatography-Mass Spectrometer) analysis. Experimental results reflected, that the backbone of CAL-n comprised (1 → 2), (1 → 6), (1 → 3) beta-pyran glycoside bond, without (1 → 4) beta-pyran glycoside bond. In addition, an MTT assay indicated that the growth of HepG₂ cells was affected by CAL-n, with a concentration dependant ration. The results indicated that CAL-n should by exploration as anti-tumor activities in vivo.

Keywords: Antitumor activity; Crinum asiaticum L; Polysaccharides; Structure.