Site-directed RNA editing (SDRE): Off-target effects and their countermeasures

Shaoshuai Mao; Yajing Liu; Shisheng Huang; Xingxu Huang; Tian Chi

doi:10.1016/j.jgg.2019.11.005

Site-directed RNA editing (SDRE): Off-target effects and their countermeasures

J Genet Genomics. 2019 Nov 20;46(11):531-535. doi: 10.1016/j.jgg.2019.11.005. Epub 2019 Nov 27.

Authors

Shaoshuai Mao¹, Yajing Liu¹, Shisheng Huang¹, Xingxu Huang², Tian Chi³

Affiliations

¹ School of Life Sciences and Technology, ShanghaiTech University, Shanghai, 201210, China; CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, 200031, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
² School of Life Sciences and Technology, ShanghaiTech University, Shanghai, 201210, China.
³ School of Life Sciences and Technology, ShanghaiTech University, Shanghai, 201210, China; Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06520, USA. Electronic address: chitian@shanghaitech.edu.cn.

PMID: 31889638
DOI: 10.1016/j.jgg.2019.11.005

Abstract

Site-directed RNA editing (SDRE) is invaluable to basic research and clinical applications and has emerged as a new frontier in genome editing. The past few years have witnessed a surge of interest in SDRE, with SDRE tools emerging at a breathtaking pace. However, off-target effects of SDRE remain a tough problem, which constitutes a major hurdle to their clinical applications. Here we discuss the diverse strategies for combating off-target editing, drawing lessons from the published studies as well as our ongoing research. Overall, SDRE is still at its infancy, with significant challenges and exciting opportunities ahead.

Keywords: Cas13; Gene editing; Off-target; RNA editing; Specificity.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

CRISPR-Cas Systems / genetics
Gene Editing
RNA Editing / genetics*
RNA, Guide, CRISPR-Cas Systems