Immunomodulatory properties of photopolymerizable fucoidan and carrageenans

Md Lutful Amin; Damia Mawad; Socrates Dokos; Pramod Koshy; Penny Jo Martens; Charles C Sorrell

doi:10.1016/j.carbpol.2019.115691

Immunomodulatory properties of photopolymerizable fucoidan and carrageenans

Carbohydr Polym. 2020 Feb 15:230:115691. doi: 10.1016/j.carbpol.2019.115691. Epub 2019 Nov 28.

Authors

Md Lutful Amin¹, Damia Mawad², Socrates Dokos³, Pramod Koshy⁴, Penny Jo Martens³, Charles C Sorrell⁵

Affiliations

¹ School of Materials Science and Engineering, UNSW Sydney, Sydney, NSW 2052, Australia; Graduate School of Biomedical Engineering, UNSW Sydney, Sydney, NSW 2052, Australia. Electronic address: m.amin@student.unsw.edu.au.
² School of Materials Science and Engineering, UNSW Sydney, Sydney, NSW 2052, Australia; Centre for Advanced Macromolecular Design, UNSW Sydney, Sydney, NSW 2052, Australia; Australian Centre for NanoMedicine and ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, UNSW Sydney, Sydney, NSW 2052, Australia. Electronic address: damia.mawad@unsw.edu.au.
³ Graduate School of Biomedical Engineering, UNSW Sydney, Sydney, NSW 2052, Australia.
⁴ School of Materials Science and Engineering, UNSW Sydney, Sydney, NSW 2052, Australia.
⁵ School of Materials Science and Engineering, UNSW Sydney, Sydney, NSW 2052, Australia. Electronic address: c.sorrell@unsw.edu.au.

PMID: 31887956
DOI: 10.1016/j.carbpol.2019.115691

Abstract

Innovative approaches to the control of immune response to tissue engineering scaffolds is of high priority. IL-10, an anti-inflammatory cytokine, has traditionally been conjugated to synthetic polymers for local immunomodulation. Marine-sulfated polysaccharides have been reported to possess anti-inflammatory properties. In the present work, it was hypothesized that photopolymerizable fucoidan and carrageenan play similar roles as the IL-10. The polysaccharides were functionalized with methacrylate groups. Their immunomodulatory properties were evaluated and compared relative to IL-10. The polysaccharides were characterized by NMR spectroscopy, revealing 12-13 % functionalization. The data revealed that fucoidan had the same activity as the IL-10 in decreasing LPS- and IFN-γ-stimulated CD86 expression. In addition, fucoidan had a protective role against LPS- and IFN-γ-induced cell growth inhibition. All polysaccharides demonstrated ∼90 % superoxide radical scavenging and they considerably decreased LPS-stimulated nitric oxide production. These results suggest that photopolymerizable fucoidan can be an alternative to IL-10 in the design of immunomodulatory biomaterials.

Keywords: Carrageenan; Fucoidan; Immunomodulatory properties; Polysaccharide; Tissue engineering.

Publication types

Comparative Study

MeSH terms

Animals
Carrageenan / pharmacology*
Cell Line
Humans
Immunologic Factors / pharmacology*
Interleukin-10 / pharmacology
Macrophages / cytology
Macrophages / drug effects*
Macrophages / immunology*
Polysaccharides / pharmacology*

Substances

IL10 protein, human
Immunologic Factors
Polysaccharides
Interleukin-10
Carrageenan
fucoidan