Degradation of polysaccharides from Sargassum fusiforme using UV/H2O2 and its effects on structural characteristics

Xiaoyong Chen; Ruifen Zhang; Yizhou Li; Xiong Li; Lijun You; Viktoryia Kulikouskaya; Kseniya Hileuskaya

doi:10.1016/j.carbpol.2019.115647

Degradation of polysaccharides from Sargassum fusiforme using UV/H₂O₂ and its effects on structural characteristics

Carbohydr Polym. 2020 Feb 15:230:115647. doi: 10.1016/j.carbpol.2019.115647. Epub 2019 Nov 19.

Authors

Xiaoyong Chen¹, Ruifen Zhang², Yizhou Li³, Xiong Li³, Lijun You⁴, Viktoryia Kulikouskaya⁵, Kseniya Hileuskaya⁵

Affiliations

¹ School of Food Science and Engineering, South China University of Technology, Guangzhou, Guangdong, 510640, China.
² Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou, Guangdong, 510610, China.
³ School of Food Science and Engineering, South China University of Technology, Guangzhou, Guangdong, 510640, China; Overseas Expertise Introduction Center for Food Nutrition and Human Health (111 Center), Guangzhou, Guangdong, 510640, China.
⁴ School of Food Science and Engineering, South China University of Technology, Guangzhou, Guangdong, 510640, China; Overseas Expertise Introduction Center for Food Nutrition and Human Health (111 Center), Guangzhou, Guangdong, 510640, China. Electronic address: feyoulijun@scut.edu.cn.
⁵ Institute of Chemistry of New Materials, National Academy of Sciences of Belarus, Minsk, 220141, Belarus.

PMID: 31887897
DOI: 10.1016/j.carbpol.2019.115647

Abstract

The depolymerization effect of UV/H₂O₂ on the polysaccharides from Sargassum fusiforme (PSF), a brown algae, were studied. The structural changes of PSF before and after UV/H₂O₂ treatment were analyzed, and molecular weight changes during in vitro digestion were determined. Results indicated that the molecular weight of PSF was reduced from ∼289 to ∼12.6 kDa within 2 h with UV/150 mmol/L H₂O₂, and the depolymerization effect of UV/H₂O₂ was significantly higher than that of UV or H₂O₂ alone. In addition, the UV/H₂O₂ treatment had a high recovery rate of total sugar (93.54 %) and clearance rate of protein (76.34 %). The monosaccharide composition showed that UV/H₂O₂ treatment could increase the mole percentage of mannose (37.44 %) and decrease the mole percentage of fucose (14.88 %). The helix-coil transition, X-ray diffraction (XRD) and atomic force microscopy (AFM) imaging showed that the UV/H₂O₂ treatment depolymerized PSF. Rheological studies indicated that PSF with UV/H₂O₂ treatment had lower viscosity. In vitro digestion showed that PSF was minimally digested with the in vitro gastrointestinal tract simulation, but PSF with UV/H₂O₂ treatment could be digested in the low acid environment in the simulated gastric juice, but was minimally digested with the simulated intestinal juice. This studied suggested that the preparation and application of functional PSF with low molecular weight might be beneficial.

Keywords: Degradation; H(2)O(2); Polysaccharides; Sargassum fusiforme; UV.