Antimicrobial properties of starch films incorporated with chitosan nanoparticles: In vitro and in vivo evaluation

Ruzanna Ahmad Shapi'i; Siti Hajar Othman; Norhazirah Nordin; Roseliza Kadir Basha; Mohd Nazli Naim

doi:10.1016/j.carbpol.2019.115602

Antimicrobial properties of starch films incorporated with chitosan nanoparticles: In vitro and in vivo evaluation

Carbohydr Polym. 2020 Feb 15:230:115602. doi: 10.1016/j.carbpol.2019.115602. Epub 2019 Nov 14.

Authors

Ruzanna Ahmad Shapi'i¹, Siti Hajar Othman², Norhazirah Nordin³, Roseliza Kadir Basha³, Mohd Nazli Naim³

Affiliations

¹ Department of Process and Food Engineering, Faculty of Engineering, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Materials Processing and Technology Laboratory, Institute of Advanced Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
² Department of Process and Food Engineering, Faculty of Engineering, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Materials Processing and Technology Laboratory, Institute of Advanced Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. Electronic address: s.hajar@upm.edu.my.
³ Department of Process and Food Engineering, Faculty of Engineering, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.

PMID: 31887886
DOI: 10.1016/j.carbpol.2019.115602

Abstract

Chitosan nanoparticles (CNP) were synthesized via ionic gelation and used for the preparation of starch-based nanocomposite films containing different concentration of CNP (0, 5, 10, 15, 20% w/w). Antimicrobial properties of starch/CNP films was evaluated via in vitro (disc diffusion analysis) and in vivo (microbial count in wrapped cherry tomatoes) study. It was found that inhibitory zone of the 15 and 20% of starch/CNP films were clearly observed for all the tested bacteria including Bacillus cereus, Staphylococcus aureus, Escherichia coli and Salmonella typhimurium. In vivo study revealed that the starch/CNP film (15% w/w) was more efficient to inhibit the microbial growth in cherry tomatoes (7 × 10² CFU/g) compared to neat starch film (2.15 × 10³ CFU/g) thus confirmed the potential application of the films as antimicrobial food packaging.

Keywords: Active packaging; Antimicrobial agent; Biopolymer; Cherry tomato; Chitosan; Starch film.

MeSH terms

Anti-Infective Agents / chemistry*
Anti-Infective Agents / pharmacology
Chitosan / analogs & derivatives*
Edible Films*
Food Packaging / methods
Gram-Negative Bacteria / drug effects
Gram-Positive Bacteria / drug effects
Nanoparticles / chemistry*
Starch / chemistry*

Substances

Anti-Infective Agents
Starch
Chitosan