A sensitive accurate spectrofluorimetric technique was developed to detect cefadroxil and cefradine traces in water samples simultaneously, by applying a procedure based on the formation of hydrolysis products corresponding to these compounds by sodium hydroxide (1 N NaOH) treatment. The conventional and the synchronous fluorescence spectra of these hydrolyzed products were totally overlapped making resolving of this mixture impossible. The second-derivative constant-wavelength synchronous fluorescence spectra allowed their detection simultaneously in a single scan after experimental conditions optimization, which was measured at 390 nm and 379 nm for cefadroxil and cefradine, respectively at Δλ = 30.0. The calibration curves between derivative synchronous fluorescence intensity and drugs concentration showed suitable linear correlation in the range of 0.1 to 5 μg.mL-1 for cefadroxil and 0.5-10 μg.mL-1 for cefradine. The proposed fluorimetric method is superior in being simple, environmental friendly and cost effective in comparison to the previously published reported methods.
Keywords: Cefadroxil; Cefradine and water samples; Derivative synchronous spectrofluorimetry.
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