Experimental supporting data on DIS3L2 over nonsense-mediated mRNA decay targets in human cells

Paulo J da Costa; Juliane Menezes; Margarida Saramago; Juan F García-Moreno; Hugo A Santos; Margarida Gama-Carvalho; Cecília M Arraiano; Sandra C Viegas; Luísa Romão

doi:10.1016/j.dib.2019.104943

Experimental supporting data on DIS3L2 over nonsense-mediated mRNA decay targets in human cells

Data Brief. 2019 Dec 6:28:104943. doi: 10.1016/j.dib.2019.104943. eCollection 2020 Feb.

Authors

Affiliations

¹ Department of Human Genetics, Instituto Nacional de Saúde Doutor Ricardo Jorge, Lisboa, Portugal.
² University of Lisbon, Faculty of Sciences, BioISI - Biosystems and Integrative Sciences Institute, Lisboa, Portugal.
³ Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras, Portugal.

Abstract

In this article, we present supportive data related to the research article "A role for DIS3L2 over natural nonsense-mediated mRNA decay targets in human cells" [1], where interpretation of the data presented here is available. Indeed, here we analyze the impact of the DIS3L2 exoribonuclease over nonsense-mediated mRNA decay (NMD)-targets. Specifically, we present data on: a) the expression of various reporter human β-globin mRNAs, monitored by Northern blot and RT-qPCR, before and after altering DIS3L2 levels in HeLa cells, and b) the gene expression levels of deregulated transcripts generated by re-analyzing publicly available data from UPF1-depleted HeLa cells that were further cross-referenced with a dataset of transcripts upregulated in DIS3L2-depleted cells. These analyses revealed that DIS3L2 regulates the levels of a subset of NMD-targets. These data can be valuable for researchers interested in the NMD mechanism.

Keywords: DIS3L2; NMD; NMD-targets; UPF1; mRNA degradation; mRNA surveillance.