Study on Pharmacokinetic and Bioavailability of Tamarixetin after Intravenous and Oral Administration to Rats

Jiayuan Shen; Qi Jia; Xuhua Huang; Guangzhe Yao; Wenjuan Ma; Yanxu Chang; Huizi Ouyang; Jun He

doi:10.1155/2019/6932053

Study on Pharmacokinetic and Bioavailability of Tamarixetin after Intravenous and Oral Administration to Rats

Evid Based Complement Alternat Med. 2019 Dec 10:2019:6932053. doi: 10.1155/2019/6932053. eCollection 2019.

Authors

Jiayuan Shen^{1

2}, Qi Jia², Xuhua Huang², Guangzhe Yao², Wenjuan Ma², Yanxu Chang², Huizi Ouyang^{1

2}, Jun He²

Affiliations

¹ First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
² Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China.

Abstract

In this study, a sensitive and reliable HPLC-MS/MS method was established to quantify tamarixetin in rat plasma. This method was then applied to research on the pharmacokinetic and bioavailability of tamarixetin after intravenous and oral administration in vivo. The study was performed on CORTECS C₁₈ column (4.6 mm × 150 mm, 2.7 μm) using mobile phase composed of methanol-water-formic acid (85 : 15 : 0.1, v/v) at a flow rate of 0.3 mL/min by a tandem mass system with an electrospray ionization (ESI) source in the negative multiple-reaction monitoring (MRM) mode. The calibration curves showed good linearity in the range of 5-4000 ng/mL. The intra- and interday precision of tamarixetin was less than 8.7% and 4.8%, respectively, and accuracy was within ±9.5%. Extraction recovery (91.4-100.0%) and matrix effect (99.4-107.4%) met the guidelines published by regulatory authorities. The oral bioavailability of tamarixetin was 20.3 ± 12.4%.