Oxidative enzymes from newly local strain Aspergillus iizukae EAN605 using pumpkin peels as a production substrate: Optimized production, characterization, application and techno-economic analysis

Efaq Noman; A A Al-Gheethi; Balkis A Talip; Radin Mohamed; Amir Hashim Kassim

doi:10.1016/j.jhazmat.2019.121954

Oxidative enzymes from newly local strain Aspergillus iizukae EAN605 using pumpkin peels as a production substrate: Optimized production, characterization, application and techno-economic analysis

J Hazard Mater. 2020 Mar 15:386:121954. doi: 10.1016/j.jhazmat.2019.121954. Epub 2019 Dec 23.

Authors

Efaq Noman¹, A A Al-Gheethi², Balkis A Talip³, Radin Mohamed⁴, Amir Hashim Kassim⁴

Affiliations

¹ Department of Applied Microbiology, Faculty of Applied Sciences, Taiz University, Taiz Yemen; Faculty of Applied Sciences and Technology, Universiti Tun Hussein Onn Malaysia (UTHM), KM11 Jalan Panchor, 84000 Pagoh, Muar, Johor, Malaysia.
² Micro-pollutant Research Centre (MPRC), Department of Water and Environmental Engineering, Faculty of Civil & Environmental Engineering, Universiti Tun Hussein Onn Malaysia, 86400 Parit Raja, Batu Pahat, Johor, Malaysia. Electronic address: adel@uthm.edu.my.
³ Faculty of Applied Sciences and Technology, Universiti Tun Hussein Onn Malaysia (UTHM), KM11 Jalan Panchor, 84000 Pagoh, Muar, Johor, Malaysia. Electronic address: balkis@uthm.edu.my.
⁴ Micro-pollutant Research Centre (MPRC), Department of Water and Environmental Engineering, Faculty of Civil & Environmental Engineering, Universiti Tun Hussein Onn Malaysia, 86400 Parit Raja, Batu Pahat, Johor, Malaysia.

PMID: 31884363
DOI: 10.1016/j.jhazmat.2019.121954

Abstract

The present study deals with optimizing, producing, characterizing, application and techno- economic analysis of oxidative enzymes [Laccase (Lac), manganese peroxidase (MnP), and lignin peroxidase (LiP)] from Aspergillus iizukae EAN605 in submerged fermentation process using pumpkin peels as a production substrate. The best operating parameters for producing Lac, MnP and LiP (6.15, 2.58 and 127.99 U mg^-1 respectively) were recorded with 20 g 100 mL^-1 of substrate, 4.6 mL 100 mL^-1 of inoculum size at pH 5.5 after 10 days. The crude enzyme exhibited high stability at pH (3-9) and temperatures (20-60 °C). K_m (Michaelis-Menten) of Lac, MnP and LiP crude enzyme was 2.25, 1.79 and 0.72 mM respectively. The decolourization of Remazol Brilliant Blue R by the crude enzyme was 84.84 %. The techno-economic analysis was assessed for a production unit with an annual operating time for enzymatic production and application is 7920 h/year and 100 m³ of the capacity. The process would produce 27,000 cm³ of crude enzyme with a price of USD 0.107 per cm³ compared to USD 1 per cm³ of the current commercial enzyme. The findings indicated that pumpkin peels have potential as a production substrate for oxidative enzymes from A. iizukae EAN605 and is economically feasible.

Keywords: Decolourization; Laccase; Lignin peroxidase; Manganese peroxidase; Response surface methodology; Submerged fermentation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aspergillus / enzymology*
Bioreactors / economics
Bioreactors / microbiology*
Cellulose / chemistry
Cost-Benefit Analysis
Cucurbita / chemistry*
Fermentation
Laccase / isolation & purification*
Peroxidases / isolation & purification*
Substrate Specificity

Substances

Cellulose
Laccase
Peroxidases
lignin peroxidase
manganese peroxidase

Supplementary concepts

Aspergillus iizukae