Validation of methylene blue viability staining with the emerging pathogen Candida auris

Ryan A Parker; Kyle T Gabriel; Kayla Graham; Christopher T Cornelison

doi:10.1016/j.mimet.2019.105829

Validation of methylene blue viability staining with the emerging pathogen Candida auris

J Microbiol Methods. 2020 Feb:169:105829. doi: 10.1016/j.mimet.2019.105829. Epub 2019 Dec 27.

Authors

Ryan A Parker¹, Kyle T Gabriel¹, Kayla Graham¹, Christopher T Cornelison²

Affiliations

¹ BioInnovation Laboratory, Kennesaw State University, Kennesaw, GA 30144, USA.
² BioInnovation Laboratory, Kennesaw State University, Kennesaw, GA 30144, USA. Electronic address: ccornel5@kennesaw.edu.

PMID: 31884053
DOI: 10.1016/j.mimet.2019.105829

Abstract

Methylene blue viability staining has been traditionally used to assess viability of Saccharomyces cerevisiae in brewing and wine making. Here, this method was tested and validated with the emerging fungal pathogen Candida auris to determine if this species would also deferentially stain, which could provide utility in assessing microbial control and disinfectant efficacy.

Publication types

Validation Study

MeSH terms

Candida / cytology*
Candidiasis / diagnosis
Cell Count / methods
Cell Survival / physiology
Humans
Methylene Blue / pharmacology*
Staining and Labeling / methods*

Substances

Methylene Blue