Preparation of graphene oxide incorporated monolithic chip based on deep eutectic solvents for solid phase extraction

Abstract

A monolithic chip incorporated with graphene oxide (GO) based on deep eutectic solvents (DESs) was developed for solid phase extraction (SPE). The carboxylated GO was modified with p-aminostyrene (pAS) by amidation, and the obtained GO (pAS-COOH-GO) was covalently incorporated into poly(butyl methacrylate-co-ethylene glycol dimethyl methacrylate) monolithic chip. Due to the high viscosity characteristics of DESs, a uniform pAS-COOH-GO incorporated monolithic chip with good permeability can be achieved. A systematic study of the preparation parameters on the performance of the resulting monolith was carried out, including the content of pAS-COOH-GO, DESs composition and porogen ratio. The resulting pAS-COOH-GO incorporated monolith was characterized by nitrogen adsorption, scanning electron microscopy, and transmission electron microscopy. The GO-doped monolithic chip can be used for SPE of polycyclic aromatic hydrocarbons (phenanthrene, anthracene, fluoranthene, pyrene, benzo(a)anthracene, benzo(a) pyrene, dibenzo(a,h)anthracene and inden(1,2,3,-cd)pyrene). The recoveries were all higher than 90%, which was about twice as high as that of the monolithic chip prepared without GO. By comparing the SPE results with that of GO free monolithic chip, good enrichment effects were demonstrated on the pAS-COOH-GO incorporated monolithic chip.

Keywords: Deep eutectic solvents; Graphene oxide; Microfluidic chip; Polycyclic aromatic hydrocarbons; Solid phase extraction.