DNA assembly is the core technology of synthetic biology. With the development of synthetic biology, researchers have developed different DNA assembly technologies that rely on DNA polymerase or DNA ligase, and also have developed some non-enzyme-dependent DNA assembly techniques to facilitate the automation of DNA assembly. The assembly of large fragments of DNA from a few hundred kb to Mb is mostly dependent on microbial recombination. In this paper, the three types of DNA assembly technologies, including enzyme-dependent, non-enzymatic and in vivo homologous recombination, are reviewed.
DNA 组装是合成生物学研究的核心技术。随着合成生物学的发展，研究者开发了依赖于DNA 聚合酶或DNA 连接酶的不同DNA 组装技术；为了降低组装成本和便于实现DNA 组装的自动化，也发展了一些非酶依赖的DNA 组装技术；而几百kb 到Mb 的大片段DNA 的组装则多数依赖于微生物体内重组。文中主要综述了酶依赖、非酶依赖和体内同源重组三类DNA 组装技术及其发展情况。.
Keywords: DNA assembly; enzyme-dependent; homologous recombination; synthesis; synthetic biology.