In Situ Neutralization Protocols for Boc-SPPS

Ramkrishna Adhikary; Philip E Dawson

doi:10.1007/978-1-0716-0227-0_3

In Situ Neutralization Protocols for Boc-SPPS

Methods Mol Biol. 2020:2103:29-40. doi: 10.1007/978-1-0716-0227-0_3.

Authors

Ramkrishna Adhikary¹, Philip E Dawson²

Affiliations

¹ Department of Chemistry, The Scripps Research Institute, La Jolla, CA, USA.
² Department of Chemistry, The Scripps Research Institute, La Jolla, CA, USA. dawson@scripps.edu.

PMID: 31879917
DOI: 10.1007/978-1-0716-0227-0_3

Abstract

Significant effort has been devoted to the optimization of solid-phase peptide synthesis (SPPS) to maximize the process to facilitate the synthesis of a desired peptide sequence, without extensive optimization or resynthesis. Over the last 25 years, a set of synthetic protocols developed by Kent and Alewood has proven to be robust and efficient for Boc/Bzl SPPS and has been widely adopted by the research community. In this chapter, we describe a variation of manual in situ neutralization protocols for Boc-SPPS that are highly effective for the rapid synthesis of peptides with different C-terminal functionalities.

Keywords: Aggregation; Boc-SPPS; Long peptides; Rapid synthesis; Solid-phase peptide synthesis.

MeSH terms

Chemistry Techniques, Synthetic
Esters / chemistry
Neutralization Tests / methods*
Peptides / chemical synthesis*
Solid-Phase Synthesis Techniques / methods*
Sulfhydryl Compounds / chemistry

Substances

Esters
Peptides
Sulfhydryl Compounds