Data on dysfunctional muscle contraction and genes contractile expression associated with chlorpyrifos exposure in slow twitch skeletal muscle

Nancy Hallal; Mahmoud Khalil; Mohamed E Moustafa; Wiam Ramadan; Wissam H Joumaa

doi:10.1016/j.dib.2019.104775

Data on dysfunctional muscle contraction and genes contractile expression associated with chlorpyrifos exposure in slow twitch skeletal muscle

Data Brief. 2019 Nov 9:27:104775. doi: 10.1016/j.dib.2019.104775. eCollection 2019 Dec.

Authors

Nancy Hallal¹, Mahmoud Khalil¹, Mohamed E Moustafa^{1

2}, Wiam Ramadan^{3

4}, Wissam H Joumaa³

Affiliations

¹ Department of Biological Sciences, Faculty of Science, Beirut Arab University, Lebanon.
² Department of Biochemistry, Faculty of Sciences, Alexandria University, Egypt.
³ Rammal Hassan Rammal Research Laboratory, PhyToxE Research Group, Faculty of Sciences, Lebanese University, Nabatieh, Lebanon.
⁴ Lebanese Institute for Biomedical Research and Application (LIBRA), Lebanese International University (LIU), Beirut, Lebanon.

Abstract

Chlorpyrifos (CPF) is a toxic organophosphate commonly used worldwide. Its residues are being detected in different environmental matrixes and hence in the food chain. Repeated CPF exposure might pose health risk for the general population on long term. This data article contains the data of contractility impairment further to dietary exposure to CPF on a hind limb skeletal muscle; soleus, a typical slow twitch skeletal muscle. Thirty adult male rats Sprague Dawley are divided into three groups receiving the following daily diet for 6 weeks: Group 1 (vehicle), Group 2: CPF1 (CPF 1mg/kg/day) and Group 3: CPF5 (CPF 5 mg/kg/day). Soleus twitch tension and fatigability index are determined at the end of the treatment. The activity of acteylcholinesterase enzyme is assessed in the tissues homogenate. Additionally, we examined the expression levels of ryanodine type 1 receptor (RyR1), ATPase Sarcoplasmic/Endoplasmic Reticulum Ca²⁺ Transporting 1 (Atp2a1), ATPase Sarcoplasmic/Endoplasmic Reticulum Ca²⁺ Transporting 2 (Atp2a2) and nicotinic acetylcholine receptor (nAChR) in CPF-exposed skeletal muscle tissue using quantitative real time polymerase chain reaction. CPF exposure at two different doses induced an increase in twitch contraction in soleus muscle along with an increase in fatigability index. These increases are accompanied by low level of acetylcholinesterase enzyme activity as well as modification in genes level expression of nAChR, RyR1, Atp2a1 and Atp2a2 involved in contractility.

Keywords: Acetylcholinesterase; Chlorpyrifos; Contractility; Nicotinic acetylcholine receptor; Ryanodine receptor; Sarco/endoplasmic reticulum Ca2+-ATPase; Soleus.