Capping 1,3-propanedithiol to boost the antibacterial activity of protein-templated copper nanoclusters

Amit Nain; Yu-Ting Tseng; Shih-Chun Wei; Arun Prakash Periasamy; Chih-Ching Huang; Fan-Gang Tseng; Huan-Tsung Chang

doi:10.1016/j.jhazmat.2019.121821

Capping 1,3-propanedithiol to boost the antibacterial activity of protein-templated copper nanoclusters

J Hazard Mater. 2020 May 5:389:121821. doi: 10.1016/j.jhazmat.2019.121821. Epub 2019 Dec 9.

Authors

Amit Nain¹, Yu-Ting Tseng², Shih-Chun Wei², Arun Prakash Periasamy², Chih-Ching Huang³, Fan-Gang Tseng⁴, Huan-Tsung Chang⁵

Affiliations

¹ Institute of Physics, Academia Sinica, Taipei, 11529, Taiwan; Nano Science and Technology Program, Taiwan International Graduate Program, Institute of Physics, Academia Sinica, Taipei, 11529, Taiwan; Department of Engineering and System Science, National Tsing Hua University, Hsinchu, 30013, Taiwan; Department of Chemistry, National Taiwan University, Taipei, 10617, Taiwan.
² Department of Chemistry, National Taiwan University, Taipei, 10617, Taiwan.
³ Department of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung, 20224, Taiwan; School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, 80708, Taiwan. Electronic address: huanging@ntou.edu.tw.
⁴ Department of Engineering and System Science, National Tsing Hua University, Hsinchu, 30013, Taiwan; Research Center for Applied Sciences Academia Sinica, Taipei, 11529, Taiwan; Frontier Research Center on Fundamental and Applied Sciences of Matters, National Tsing Hua University, Hsinchu, 30013, Taiwan. Electronic address: fangang@ess.nthu.edu.tw.
⁵ Department of Chemistry, National Taiwan University, Taipei, 10617, Taiwan; Department of Chemistry, Chung Yuan Christian University, Chungli District, Taoyuan City, 32023, Taiwan. Electronic address: changht@ntu.edu.tw.

PMID: 31879116
DOI: 10.1016/j.jhazmat.2019.121821

Abstract

We have prepared copper nanoclusters (Cu NCs) in the presence of bovine serum albumin (BSA) and 1,3-propanedithiol (PDT). The PDT/BSA-Cu NCs possess great activities against different types of bacteria, including non-multidrug-resistant bacteria (Escherichia coli, Salmonella Enteritidis, Pseudomonas aeruginosa, and Staphylococcus aureus) and multidrug-resistant bacteria (methicillin-resistant S. aureus). Their minimal inhibitory concentration (MIC) values are at least 242-fold and 10-fold lower than that of the free PDT and BSA-Cu NCs, respectively. The PDT/BSA-Cu NCs are strongly bound to the bacterial membrane, in which they induce the generation of ascorbyl (Asc) and perhydroxyl (HOO) radicals that result in disruption of their membrane integrity. At a concentration of 100-fold higher than their MIC for Escherichia coli, the PDT/BSA-Cu NCs exhibit negligible cytotoxicity towards the tested mammalian cells and show insignificant hemolysis. We have further demonstrated that low-cost PDT/BSA-Cu NCs-coated carbon fiber fabrics (CFFs) are effective against antibacterial growth, showing their great potential for antifouling applications.

Keywords: Antibacterial textiles; Copper nanoclusters; Free radicals; Protein templates; Thiol ligands.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / chemistry
Anti-Bacterial Agents / pharmacology*
Carbon Fiber / chemistry
Carbon Fiber / microbiology
Copper / chemistry*
Gram-Negative Bacteria / drug effects
Gram-Positive Bacteria / drug effects
Microbial Sensitivity Tests
Microbial Viability / drug effects
Nanostructures / chemistry*
Propane / analogs & derivatives*
Propane / chemistry
Serum Albumin, Bovine / chemistry*
Sulfhydryl Compounds / chemistry*

Substances

Anti-Bacterial Agents
Carbon Fiber
Sulfhydryl Compounds
Serum Albumin, Bovine
Copper
1,3-propanedithiol
Propane