Shell-vial Assay in Diagnosis of Disseminated BCG Infection in an Immunodeficient Child

Mustapha Fellag; Jamal Saad; Vincent Barlogis; Gérard Michel; Michel Drancourt

doi:10.1097/INF.0000000000002565

Shell-vial Assay in Diagnosis of Disseminated BCG Infection in an Immunodeficient Child

Pediatr Infect Dis J. 2020 Mar;39(3):258-259. doi: 10.1097/INF.0000000000002565.

Authors

Mustapha Fellag^{1

2}, Jamal Saad^{1

2}, Vincent Barlogis³, Gérard Michel³, Michel Drancourt^{1

2}

Affiliations

¹ From the Aix Marseille University, MEPHI, IRD.
² IHU-Méditerranée Infection, Marseille, France.
³ Pediatric Oncology Department, Assistance Publique-hôpitaux de Marseille, Marseille, France.

PMID: 31876612
DOI: 10.1097/INF.0000000000002565

Abstract

We are reporting a case of Bacillus Calmette-Guérin vaccine-disseminated infection in a 19-month-old HIV-negative girl diagnosed with severe combined immunodeficiency. While standard culture protocols failed to isolate and culture the Mycobacterium bovis Bacillus Calmette-Guérin strain, it was isolated from skin and mesenteric lymph node biopsies using the shell-vial assay, allowing whole-genome sequencing and in silico drug susceptibility testing.

Publication types

Case Reports
Research Support, Non-U.S. Gov't

MeSH terms

Antitubercular Agents / therapeutic use
Biopsy
Bone Marrow Transplantation / adverse effects
Bone Marrow Transplantation / methods
Drug Therapy, Combination
Female
Humans
Infant
Lymph Nodes / microbiology
Lymph Nodes / pathology
Microbial Sensitivity Tests / methods*
Mycobacterium bovis* / drug effects
Mycobacterium bovis* / genetics
Mycobacterium bovis* / immunology
Severe Combined Immunodeficiency / complications*
Severe Combined Immunodeficiency / therapy
Skin Ulcer / etiology
Skin Ulcer / pathology
Treatment Outcome
Tuberculosis / diagnosis*
Tuberculosis / drug therapy
Tuberculosis / etiology*

Substances

Antitubercular Agents