The in vitro effect of orexin a on the porcine myometrial transcriptomic profile during the early-implantation period

Kamil Dobrzyn; Marta Kiezun; Karol Szeszko; Marlena Gudelska; Katarzyna Kisielewska; Edyta Rytelewska; Ewa Zaobidna; Joanna Wyrebek; Kinga Bors; Grzegorz Kopij; Barbara Kaminska; Tadeusz Kaminski; Nina Smolinska

doi:10.1016/j.theriogenology.2019.12.004

The in vitro effect of orexin a on the porcine myometrial transcriptomic profile during the early-implantation period

Theriogenology. 2020 Feb:143:157-167. doi: 10.1016/j.theriogenology.2019.12.004. Epub 2019 Dec 10.

Authors

Affiliations

¹ Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719, Olsztyn-Kortowo, Poland. Electronic address: kamil.dobrzyn@uwm.edu.pl.
² Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719, Olsztyn-Kortowo, Poland. Electronic address: marta.kiezun@uwm.edu.pl.
³ Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719, Olsztyn-Kortowo, Poland. Electronic address: karol.szeszko@uwm.edu.pl.
⁴ Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719, Olsztyn-Kortowo, Poland. Electronic address: marlena.gudelska@uwm.edu.pl.
⁵ Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719, Olsztyn-Kortowo, Poland. Electronic address: katarzyna.kisielewska@uwm.edu.pl.
⁶ Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719, Olsztyn-Kortowo, Poland. Electronic address: edyta.rytelewska@uwm.edu.pl.
⁷ Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719, Olsztyn-Kortowo, Poland. Electronic address: ewa.zaobidna@uwm.edu.pl.
⁸ Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719, Olsztyn-Kortowo, Poland. Electronic address: joanna.wyrebek@uwm.edu.pl.
⁹ Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719, Olsztyn-Kortowo, Poland. Electronic address: kinga.bors@uwm.edu.pl.
¹⁰ Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719, Olsztyn-Kortowo, Poland. Electronic address: grzegorzkopij@gmail.com.
¹¹ Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719, Olsztyn-Kortowo, Poland. Electronic address: barbara.kaminska@uwm.edu.pl.
¹² Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719, Olsztyn-Kortowo, Poland. Electronic address: tkam@uwm.edu.pl.
¹³ Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719, Olsztyn-Kortowo, Poland. Electronic address: nina.smolinska@uwm.edu.pl.

PMID: 31875568
DOI: 10.1016/j.theriogenology.2019.12.004

Abstract

In pigs, early gestation is the most critical period deciding about the reproduction success, and it depends on many processes, involving a significant number of genes and their products. Myometrium was found to be an important source of factors pivotal for a proper course of gestation. The aim of the study was to determine the effect of orexin A (OXA) on the porcine transcriptome, and the determination of relationships among differentially expressed genes (DEG) in the porcine myometrium during implantation using microarray technology. The analyses of gene ontology (GO), DEG assays, biological pathways and networks were performed. OXA affected the expression of 461 genes with fold-change values greater than 1.2 (p < 0.05). The expression of 260 genes were up-regulated and 201 down-regulated in the OXA-treated myometrium. Twelve genes were selected for qPCR validation of differential expression based on their known role in angiogenesis, immune processes, steroid hormone signaling and prostaglandins synthesis. The analysis of relationship between DEG indicated that OXA interacts with genes involved in the inflammatory response, cytokine binding, cytokine activity, interleukin production, leukocyte migration, angiogenesis and embryonic hemopoiesis. The presented results suggest that OXA may play a key role in ensuring optimal conditions for implanting embryos.

Keywords: Implantation; Myometrium; Orexin A; Pig; Transcriptome.

MeSH terms

Animals
Female
Gene Expression Regulation / drug effects
Myometrium / drug effects*
Myometrium / metabolism*
Orexins / pharmacology*
Pregnancy
Real-Time Polymerase Chain Reaction / veterinary
Swine / physiology*
Transcriptome / drug effects*

Substances

Orexins