Assessment of Cytokine-Mediated Signaling Pathway Dysregulation in Arm Skin After CO2 Laser Therapy

Mohammad Rostami-Nejad; Mostafa Rezaei-Tavirani; Mohammad-Mehdi Zadeh-Esmaeel; Sina RezaeiTavirani; Zahra Akbari; Somayeh Esmaeili; Farshad Okhovatian

doi:10.15171/jlms.2019.42

Assessment of Cytokine-Mediated Signaling Pathway Dysregulation in Arm Skin After CO2 Laser Therapy

J Lasers Med Sci. 2019 Fall;10(4):257-263. doi: 10.15171/jlms.2019.42. Epub 2019 Oct 1.

Authors

Mohammad Rostami-Nejad¹, Mostafa Rezaei-Tavirani², Mohammad-Mehdi Zadeh-Esmaeel³, Sina RezaeiTavirani⁴, Zahra Akbari⁵, Somayeh Esmaeili⁶, Farshad Okhovatian⁷

Affiliations

¹ Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
² Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
³ Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
⁴ Proteomics Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
⁵ Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
⁶ Traditional Medicine and Materia Medica Research Center, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
⁷ Physiotherapy Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Abstract

Introduction: Laser therapy is known as an efficient approach in dermatology surgery. CO2 laser therapy is a gold standard treatment in skin surgery. This study aimed to evaluate the interferons change after CO2 laser surgery Methods: Significant differentially-expressed genes (DEGs) of arm skin after 7 days of treatment by the CO2 laser relative to the controls are downloaded from Gene Expression Omnibus (GEO) and are included in the protein-protein interaction network via a STRING database (an application of Cytoscape software). The central DEGs were identified and enriched via gene ontology by using Clue GO software. Results: A network including 78 DEGs and 100 neighbors was constructed and STAT1, MX1, ISG15, OAS1, IFIT1, IRF8, OASL, OAS2, and RSAD2 as hubs and STAT1, PTPRC, MX1, IRF8, ISG15, IL6, RORC, SAMSN1, and IFIT1 as bottlenecks were introduced. The cytokine-mediated signaling pathway, interferon gamma signaling, hepatitis C, interferon alpha/beta signaling, and the type I interferon signaling pathway were identified as 5 clusters of biological terms which are related to the central nodes. Conclusion: It can be concluded that the cytokine-mediated signaling pathway is the major pathway that is dysregulated after laser application in the treated skin.

Keywords: Gene; Interferon; Laser CO2; Network; Skin.