Multiparametric magnetic resonance imaging shows promising results to assess renal transplant dysfunction with fibrosis

Abstract

Here we assessed the diagnostic value of a quantitative multiparametric magnetic resonance imaging (mpMRI) protocol for evaluation of renal allograft dysfunction with fibrosis. Twenty-seven renal transplant patients, including 15 with stable functional allografts (eGFR mean 71.5 ml/min/1.73m²), and 12 with chronic dysfunction/established fibrosis (eGFR mean 30.1 ml/min/1.73m²), were enrolled in this prospective single-center study. Sixteen of the patients had renal biopsy (mean 150 days) before the MRI. All patients underwent mpMRI at 1.5T including intravoxel-incoherent motion diffusion-weighted imaging, diffusion tensor imaging, blood oxygen level dependent (BOLD R₂*) and T₁ quantification. True diffusion D, pseudodiffusion D*, perfusion fraction PF, apparent diffusion coefficient (ADC), fractional anisotropy (FA), R₂* and T₁ were calculated for cortex and medulla. ΔT₁ was calculated as (100x(T₁ Cortex-T₁ Medulla)/T₁ Cortex). Test-retest repeatability and inter-observer reproducibility were assessed in four and ten patients, respectively. mpMRI parameters had substantial test-retest and interobserver repeatability (coefficient of variation under 15%), except for medullary PF and D* (coefficient of variation over 25%). Cortical ADC, D, medullary ADC and ΔT₁ were all significantly decreased, while cortical T₁ was significantly elevated in fibrotic allografts. Cortical T₁ showed positive correlation to the Banff fibrosis and tubular atrophy scores. The combination of ΔT₁ and cortical ADC had excellent cross-validated diagnostic performance for detection of chronic dysfunction with fibrosis. Cortical ADC and T₁ had good performance for predicting eGFR decline at 18 months (4 or more ml/min/1.73m²/year). Thus, the combination of cortical ADC and T₁ measurements shows promising results for the non-invasive assessment of renal allograft histology and outcomes.

Keywords: T(1); apparent diffusion coefficient; eGFR slope; multiparametric MRI; renal allograft chronic dysfunction; renal allograft fibrosis.