Objective: To investigate the expression level of antisense transcript of pseudogene, general transcription factor Ⅱi psedugen23 (GTF2IP23), in breast cancer and its effect on the host gene general transcription factor Ⅱi (GTF2I). Methods: The expressions of GTF2IP23 and GTF2I were detected in 40 cases of invasive breast cancer tumors and their counterparts by using quantitative real-time polymerase chain reaction (qRT-PCR). The effects of GTF2IP23 on the expression of GTF2I gene and cell proliferation and migration were analyzed by overexpression of GTF2IP23 in breast cancer cells. Results: The expression of GTF2IP23 mRNA in breast cancer tissues was significantly higher than that in adjacent tissues (P<0.001), while the expression of GTF2I mRNA was significantly lower than that in adjacent tissues (P=0.007). The expression of GTF2IP23 was negatively correlated with GTF2I (r=-0.335, P=0.025). The expression of GTF2IP23 in breast cancer cells was significantly higher than in normal breast cells (P<0.01), while GTF2I expression in breast cancer cells was significantly lower than that in normal breast cells (P<0.01). Overexpression of GTF2IP23 in ZR-75-30 cells significantly reduced the expression of GTF2I (P=0.034) and enhanced cell proliferation (P=0.017) and migration (P=0.026) capacity. Conclusions: GTF2IP23 is distinctly upregulated in breast cancer, it inhibits the expression of real gene GTF2I and promotes the proliferation of breast cancer cells.
目的： 探讨通用转录因子Ⅱi假基因23(GTF2IP23)在乳腺癌中的表达情况以及对其真基因通用转录因子Ⅱi(GTF2I)表达和乳腺癌细胞增殖能力的影响。 方法： 应用实时荧光定量PCR法检测2012年4月至10月在新乡市中心医院行手术切除治疗的40例浸润性乳腺癌患者的肿瘤组织和对应的正常乳腺组织及乳腺癌细胞中GTF2IP23和GTF2I基因的表达情况，分析GTF2IP23在正常乳腺细胞和乳腺癌细胞中对GTF2I基因表达情况的影响以及对乳腺癌细胞增殖和迁移能力的作用。 结果： 实时荧光定量PCR法检测结果显示，乳腺癌组织中GTF2IP23 mRNA的相对表达量为9.446±0.548，明显高于癌旁组织(6.413±0.488，P<0.001)；乳腺癌组织中GTF2I mRNA的相对表达量为11.417±0.873，明显低于癌旁组织(13.659±0.517，P＝0.007)。GTF2IP23 mRNA在乳腺癌和正常组织中的表达均明显低于GTF2I mRNA；Pearson相关分析显示，GTF2IP23与GTF2I mRNA的表达呈负相关(r＝－0.335，P＝0.025)。GTF2IP23 mRNA在7株人乳腺癌细胞系中的表达水平均高于正常人乳腺细胞MCF10A(P<0.01)；而GTF2I mRNA在7株人乳腺癌细胞系的表达水平均低于正常人乳腺细胞MCF10A(P<0.01)。与转染空载体对照的ZR－75－30细胞相比，转染GTF2IP23基因的ZR－75－30细胞中GTF2I基因的mRNA相对表达量明显降低。与ZR－75－30/pcDNA3.1组比较，ZR－75－30/pcDNA3.1－GTF2IP23组细胞24 h后的增殖能力明显增强(P<0.05)。ZR－75－30/pcDNA3.1组细胞的迁移数目为(103.47±21.52)个/视野，与ZR－75－30/pcDNA3.1－GTF2IP23组[(257.23±32.39)个/视野]比较，差异有统计学意义(P<0.05)。 结论： 乳腺癌中特异性高表达的假基因GTF2IP23对其真基因GTF2I的表达有抑制作用，对乳腺癌细胞的增殖能力有一定的促进作用。.
Keywords: Breast neoplasms; GTF2I; GTF2IP23; Migration; Proliferation.