Maximizing enzymatic hydrolysis efficiency of bamboo with a mild ethanol-assistant alkaline peroxide pretreatment

Chen Huang; Guigan Fang; Longxiang Yu; Yang Zhou; Xianzhi Meng; Yongjun Deng; Kuizhong Shen; Arthur J Ragauskas

doi:10.1016/j.biortech.2019.122568

Maximizing enzymatic hydrolysis efficiency of bamboo with a mild ethanol-assistant alkaline peroxide pretreatment

Bioresour Technol. 2020 Mar:299:122568. doi: 10.1016/j.biortech.2019.122568. Epub 2019 Dec 7.

Authors

Chen Huang¹, Guigan Fang², Longxiang Yu³, Yang Zhou³, Xianzhi Meng⁴, Yongjun Deng⁵, Kuizhong Shen⁵, Arthur J Ragauskas⁶

Affiliations

¹ Institute of Chemical Industry of Forest Products, Chinese Academy of Forestry, Nanjing 210042, China; Co-Innovation Center for Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210037, China; Department of Chemical and Biomolecular Engineering, University of Tennessee Knoxville, Knoxville, TN 37996, USA.
² Institute of Chemical Industry of Forest Products, Chinese Academy of Forestry, Nanjing 210042, China; Co-Innovation Center for Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210037, China.
³ Co-Innovation Center for Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210037, China.
⁴ Department of Chemical and Biomolecular Engineering, University of Tennessee Knoxville, Knoxville, TN 37996, USA.
⁵ Institute of Chemical Industry of Forest Products, Chinese Academy of Forestry, Nanjing 210042, China.
⁶ Department of Chemical and Biomolecular Engineering, University of Tennessee Knoxville, Knoxville, TN 37996, USA; Department of Forestry, Wildlife, and Fisheries, Center for Renewable Carbon, The University of Tennessee Institute of Agriculture, Knoxville, TN 37996, USA; UTK-ORNL Joint Institute for Biological Science, Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA. Electronic address: aragausk@utk.edu.

PMID: 31874450
DOI: 10.1016/j.biortech.2019.122568

Abstract

To overcome the delignification saturation point in traditional alkaline hydrogen peroxide pretreatment (AHP), a powerful modified AHP delignification methodology was established by introducing ethanol into the system. The pretreatment caused significant lignin removal of bamboo at elevated pretreatment temperature with the highest lignin removal reaching 80.0% at 100 °C, higher than that (74.9% lignin removal) in pretreatment without the ethanol assistance. In addition, a certain amount of carbohydrates was also solubilized during the process whose recovery was 83.3% (glucan) and 67.6% (hemicellulose), respectively. The pretreated solid exhibited excellent enzymatic digestibility, with hydrolysis yields of ~100% and 95.7% for glucan and xylan, respectively. Our studies further indicate that this delignification methodology is versatile for hardwood and herbaceous plants, but does not perform well on softwood.

Keywords: Alkaline hydrogen peroxide; Bamboo; Delignification; Enzymatic hydrolysis; Low-temperature pretreatment.

MeSH terms

Ethanol*
Glucans
Hydrogen Peroxide
Hydrolysis
Lignin*

Substances

Glucans
Ethanol
Lignin
Hydrogen Peroxide